植物乳杆菌 YKX 对 的抑菌活性作用模式
The Antibacterial Activity Mode of Action of Plantaricin YKX against .
机构信息
Northwest Institute of Plateau Biology, Chinese Acadamy of Science, Xining 530001, China.
Shaanxi Province Key Laboratory of Bioresources, 2011 QinLing-Bashan Mountains Bioresources Comprehensive Development C. I. C., Qinba State Key Laboratory of Biological Resources and Ecological Environment, College of Bioscience and Bioengineering, Shaanxi University of Technology, Hanzhong 723001, China.
出版信息
Molecules. 2022 Jul 3;27(13):4280. doi: 10.3390/molecules27134280.
We aimed to evaluate the inhibitory effect and mechanism of plantaricin YKX on . The mode of action of plantaricin YKX against the cells of indicated that plantaricin YKX was able to cause the leakage of cellular content and damage the structure of the cell membranes. Additionally, plantaricin YKX was also able to inhibit the formation of biofilms. As the concentration of plantaricin YKX reached 3/4 MIC, the percentage of biofilm formation inhibition was over 50%. Fluorescent dye labeling combined with fluorescence microscopy confirmed the results. Finally, the effect of plantaricin YKX on the AI-2/LuxS QS system was investigated. Molecular docking predicted that the binding energy of AI-2 and plantaricin YKX was -4.7 kcal/mol and the binding energy of bacteriocin and luxS protein was -183.701 kcal/mol. The expression of the gene increased significantly after being cocultured with plantaricin YKX, suggesting that plantaricin YKX can affect the QS system of .
我们旨在评估植物乳酸杆菌 YKX 对 的抑制作用和机制。植物乳酸杆菌 YKX 对 细胞的作用模式表明，植物乳酸杆菌 YKX 能够导致细胞内容物泄漏，并破坏细胞膜的结构。此外，植物乳酸杆菌 YKX 还能够抑制生物膜的形成。当植物乳酸杆菌 YKX 的浓度达到 3/4 MIC 时，生物膜形成抑制率超过 50%。荧光染料标记结合荧光显微镜证实了这一结果。最后，研究了植物乳酸杆菌 YKX 对 AI-2/LuxS QS 系统的影响。分子对接预测 AI-2 和植物乳酸杆菌 YKX 的结合能为-4.7 kcal/mol，而细菌素和 luxS 蛋白的结合能为-183.701 kcal/mol。与植物乳酸杆菌 YKX 共培养后， 基因的表达显著增加，表明植物乳酸杆菌 YKX 可以影响 的 QS 系统。
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