Laboratories of Natural Substances, Pharmacology, Environment, Modeling, Health and Quality of Life (SNAMOPEQ), Faculty of Sciences, Sidi Mohamed Ben Abdellah University, Fez 30000, Morocco.
Laboratory of Biotechnology, Environment, Agri-Food and Health, Faculty of Sciences Dhar El Mahraz, Sidi Mohammed Ben Abdellah University, Fez 30050, Morocco.
Molecules. 2022 Jul 5;27(13):4322. doi: 10.3390/molecules27134322.
is among the invasive plants of Morocco; studies on its chemical composition and biological activities are few in number in Morocco. has shown molluscicidal and nematicidal and cancer-inhibitory effects, anti-inflammatory, analgesic activity, and antibacterial activity. The objective of this research is to improve this plant and assess its antibacterial and antioxidant properties as well as its total polyphenolic content (TPC) and total flavonoid content (TFC). The Folin-Ciocalteu method and the aluminium-trichloride method were used to determine TPC and TFC in hydro-ethanolic (HEE) and hydro-acetonic (HAE) leaf extract. Three assays were performed to determine the antioxidant activity: the DPPH test (radical 2,2'-diphenyl-1-picrylhydrazyl), the FRAP test (Ferric Reducing Antioxidant Power), and the TAC test. Disk diffusion and microdilution were used to test antibacterial activity against four pathogenic bacteria and . The hydro-ethanolic extract 2.54 ± 0.4 mg EAG/g has a greater polyphenol concentration than the hydro-acetonic extract 1.58 ± 0.03 mg EAG/g. Although the flavonoid content of the hydro-acetonic extract (0.067 ± 0.001 mg EQ/g) is larger than that of the hydro-ethanolic extract (0.012 ± 0.001 mg EQ/g), the flavonoid content of the hydro-ethanolic extract (0.012 ± 0.001 mg EQ/g). The DPPH values were IC-50 = 0.081 ± 0.004 mg/mL for hydro-ethanoic extract and 0.198 ± 0.019 mg/mL for hydro-acetonic extract, both extracts superior to BHT (0.122 ± 0.021 g/mL). While the FRAP assay showed a low iron-reducing power values for both extracts compared to BHT), the overall antioxidant activity of the two extracts was found to be considerable. The overall antioxidant activity of the hydro-ethanolic extract was 8.95 ± 0.42 mg EAA/g, whereas the total antioxidant activity of the hydro-acetonic extract was 6.44 ± 0.61 mg EAA/g. In comparison with the antibiotic Erythromycin, HAE and HEE from leaves demonstrated significant antibacterial action. HAE had the best inhibitory efficacy against DSM 6333, with an inhibition diameter of 10.5 ± 0.50 mm and a MIC of 7.5 ± 0.00 mg/mL, as well as against ATCC 29906, with an inhibitory diameter of 8.25 ± 0.75 mm and a MIC of 15 ± 0.00 mg/mL.
是摩洛哥的入侵植物之一；摩洛哥对其化学成分和生物活性的研究很少。已显示出杀软体动物、杀线虫和抗癌作用、抗炎、镇痛作用和抗菌活性。本研究的目的是改进这种植物，并评估其抗菌和抗氧化特性以及其总多酚含量（TPC）和总类黄酮含量（TFC）。Folin-Ciocalteu 法和三氯化铝法用于测定水-乙醇（HEE）和水-丙酮（HAE）叶提取物中的 TPC 和 TFC。进行了三种抗氧化活性测定：DPPH 试验（自由基 2,2'-二苯基-1-苦基肼）、FRAP 试验（铁还原抗氧化能力）和 TAC 试验。使用圆盘扩散和微稀释法测试了对四种致病菌和 的抗菌活性。水-乙醇提取物 2.54 ± 0.4 mg EAG/g 的多酚浓度高于水-丙酮提取物 1.58 ± 0.03 mg EAG/g。尽管水-丙酮提取物的类黄酮含量（0.067 ± 0.001 mg EQ/g）大于水-乙醇提取物（0.012 ± 0.001 mg EQ/g），但水-乙醇提取物的类黄酮含量（0.012 ± 0.001 mg EQ/g）。DPPH 值为 HEE 的 IC-50 = 0.081 ± 0.004 mg/mL，HAE 的 IC-50 = 0.198 ± 0.019 mg/mL，均优于 BHT（0.122 ± 0.021 g/mL）。尽管 FRAP 测定显示两种提取物的铁还原能力值均低于 BHT，但两种提取物的总抗氧化活性被认为相当可观。水-乙醇提取物的总抗氧化活性为 8.95 ± 0.42 mg EAA/g，而水-丙酮提取物的总抗氧化活性为 6.44 ± 0.61 mg EAA/g。与抗生素红霉素相比，叶的 HAE 和 HEE 表现出显著的抗菌作用。HAE 对 DSM 6333 的抑制效果最好，抑制直径为 10.5 ± 0.50 mm，MIC 为 7.5 ± 0.00 mg/mL，对 ATCC 29906 的抑制直径为 8.25 ± 0.75 mm，MIC 为 15 ± 0.00 mg/mL。
