Laboratory of Biotechnology Conservation and Valorisation of Natural Resources, Faculty of Sciences Dhar El Mahraz, Sidi Mohammed Ben Abdallah University, Fez 30000, Morocco.
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, P.O. Box 10219, Riyadh 11433, Saudi Arabia.
Molecules. 2022 Feb 8;27(3):1136. doi: 10.3390/molecules27031136.
This study investigated the chemical composition, antioxidant and antimicrobial activity of essential oil extracted from Lam. (EOA). Hydrodistillation was employed to extract EOA. Gas chromatography with flame ionization detection (GC-FID) and gas chromatography-mass spectrometry analyses (GC-MS) were used to determine the phytochemical composition of EOA. Antioxidant potential was examined in vitro by use of three tests: 2.2-diphenyl-1-picrilhidrazil (DPPH), ferric reducing activity power (FRAP) and total antioxidant capacity assay (TAC). Agar diffusion and microdilution bioassays were used to assess antimicrobial activity. GC/MS and GC-FID detected 34 constituents in the studied EOA. The major component was Camphor (24.97%) followed by Borneol (13.20%), 1,8 Cineol (10.88%), and Artemisia alcohol (10.20%). EOA exhibited significant antioxidant activity as measured by DPPH and FRAP assays, with IC and EC values of 0.034 ± 0.004 and 0.118 ± 0.008 mg/mL, respectively. EOA exhibited total antioxidant capacity of 7.299 ± 1.774 mg EAA/g. EOA exhibited potent antibacterial activity as judged by the low minimum inhibitory concentration (MIC) values against selected clinically-important pathogenic bacteria. MIC values of 6.568 ± 1.033, 5.971 ± 1.033, 7.164 ± 0.0 and 5.375 ± 0.0 μg/mL were observed against , , 97 and 57, respectively. EOA displayed significant antifungal activity against four strains of fungi: , , and with values of 21.50 ± 0.43, 5.31 ± 0.10, 21.50 ± 0.46 and 5.30 ± 0.036 μg/mL, respectively. The results of the current study highlight the importance of EOA as an alternative source of natural antioxidant and antibacterial drugs to combat antibiotic-resistant microbes and free radicals implicated in the inflammatory responses accompanying microbial infection.
本研究调查了 Lam.（EOA）精油的化学成分、抗氧化和抗菌活性。采用水蒸馏法提取精油。气相色谱-火焰离子化检测（GC-FID）和气相色谱-质谱分析（GC-MS）用于确定 EOA 的植物化学成分。通过使用三种测试方法：2.2-二苯基-1-苦基肼（DPPH）、铁还原活性能力（FRAP）和总抗氧化能力测定法（TAC），在体外检查抗氧化潜力。琼脂扩散和微量稀释生物测定法用于评估抗菌活性。GC/MS 和 GC-FID 在研究的 EOA 中检测到 34 种成分。主要成分是樟脑（24.97%），其次是龙脑（13.20%）、1,8 桉树脑（10.88%）和青蒿醇（10.20%）。EOA 表现出显著的抗氧化活性，如 DPPH 和 FRAP 测定所测，IC 和 EC 值分别为 0.034 ± 0.004 和 0.118 ± 0.008 mg/mL。EOA 表现出 7.299 ± 1.774 mg EAA/g 的总抗氧化能力。EOA 表现出针对选定的临床重要致病菌的低最小抑菌浓度（MIC）值，表现出很强的抗菌活性。对 、 、 97 和 57 的 MIC 值分别为 6.568 ± 1.033、5.971 ± 1.033、7.164 ± 0.0 和 5.375 ± 0.0 μg/mL。EOA 对四种真菌菌株： 、 、 和 表现出显著的抗真菌活性，值分别为 21.50 ± 0.43、5.31 ± 0.10、21.50 ± 0.46 和 5.30 ± 0.036 μg/mL。本研究的结果强调了 EOA 作为天然抗氧化和抗菌药物替代来源的重要性，以对抗抗生素耐药微生物和自由基，这些微生物和自由基与微生物感染引起的炎症反应有关。
