Laboratory of Natural Substances, Pharmacology, Environment, Modeling, Health & Quality of Life, Faculty of Sciences, Sidi Mohamed Ben Abdellah University, Fez, Morocco.
Euromed Research Center, Euromed Faculty of Pharmacy, Euromed University of Fes (UEMF) Route de Meknes, 30000, Fez, Morocco.
Sci Rep. 2023 Oct 21;13(1):18028. doi: 10.1038/s41598-023-45030-5.
The current work attempts to explore the influence of three extraction solvents on phytochemical composition, content of polyphenols, antioxidant potential, and antibacterial capacity of hydroethanolic, acetonic, and aqueous extracts from Moroccan Mentha longifolia leaves. To achieve this goal, the chemical composition was identified using an HPLC-DAD examination. The contents of polyphenols were assessed, while the total antioxidant capacity (TAC), the DPPH test, and the reducing power test (RP) were utilized to determine antioxidant capacity. To assess the antibacterial activity, the microdilution technique was carried out to calculate the minimum inhibitory (MIC) and minimum bactericidal concentrations (MBC) of extracts against four nosocomial bacteria (Bacillus cereus, Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus). Additionally, the antibacterial and antioxidant activities of all tested extracts were examined in silico against the proteins NADPH oxidase and Bacillus cereus phospholipase C. Study reveals that M. longifolia extracts contain high phenolic and flavonoids. Additionally, the hydroethanolic extract contained the highest amounts of phenolic and flavonoid content, with values of 23.52 ± 0.14 mg Gallic acid equivalent/g dry weight and 17.62 ± 0.36 mg Quercetin Equivalent/g dry weight, respectively compared to the other two extracts. The same extract showed the best antioxidant capacity (IC = 39 µg/mL ± 0.00), and the higher RP (EC of 0.261 ± 0.00 mg/mL), compared to the acetonic and aqueous extract regarding these tests. Furthermore, the hydroethanolic and acetonic extracts expressed the highest TAC (74.40 ± 1.34, and 52.40 ± 0.20 mg EAA/g DW respectively), compared with the aqueous extract. Regarding antibacterial activity, the MIC value ranges between 1.17 and 12.50 mg/mL. The in-silico results showed that the antibacterial activity of all extracts is principally attributed to kaempferol and ferulic acid, while antioxidant capacity is attributed to ferulic acid.
目前的工作试图探索三种提取溶剂对摩洛哥长叶薄荷叶片的水醇、丙酮和水提取物的植物化学成分、多酚含量、抗氧化潜力和抗菌能力的影响。为了实现这一目标，使用 HPLC-DAD 检查鉴定了化学成分。评估了多酚的含量，同时利用总抗氧化能力（TAC）、DPPH 试验和还原力试验（RP）来测定抗氧化能力。为了评估抗菌活性，采用微量稀释技术计算提取物对四种医院感染菌（蜡样芽孢杆菌、铜绿假单胞菌、大肠杆菌、金黄色葡萄球菌）的最小抑菌（MIC）和最小杀菌浓度（MBC）。此外，还在计算机上测试了所有测试提取物对 NADPH 氧化酶和蜡样芽孢杆菌磷脂酶 C 蛋白的抗菌和抗氧化活性。研究表明，长叶薄荷提取物含有高含量的酚类和类黄酮。此外，水醇提取物含有最高量的酚类和类黄酮含量，分别为 23.52 ± 0.14 mg 没食子酸当量/g 干重和 17.62 ± 0.36 mg 槲皮素当量/g 干重，高于其他两种提取物。与其他两种提取物相比，同一种提取物显示出最好的抗氧化能力（IC = 39 µg/mL ± 0.00），以及更高的 RP（EC 为 0.261 ± 0.00 mg/mL）。此外，与水提取物相比，水醇和丙酮提取物的 TAC（分别为 74.40 ± 1.34 和 52.40 ± 0.20 mg EAA/g DW）最高。关于抗菌活性，MIC 值范围在 1.17 和 12.50 mg/mL 之间。计算机模拟结果表明，所有提取物的抗菌活性主要归因于山柰酚和阿魏酸，而抗氧化能力归因于阿魏酸。
