Calugar Roxana Elena, Muntean Edward, Varga Andrei, Vana Carmen Daniela, Has Voichita Virginia, Tritean Nicolae, Ceclan Loredana Anca
Agricultural Research and Development Station Turda, Agriculturii 27, 401100 Turda, Romania.
Department of Food Science, Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 3-5 Mănăştur St., 400372 Cluj-Napoca, Romania.
Plants (Basel). 2022 Jun 21;11(13):1632. doi: 10.3390/plants11131632.
Carotenoids are important biologically active compounds in the human diet due to their role in maintaining a proper health status. Maize ( L.) is one of the main crops worldwide, in terms of production quantity, yield and harvested area, as it is also an important source of carotenoids in human nutrition worldwide. Increasing the carotenoid content of maize grains is one of the major targets of the research into maize breeding; in this context, the aim of this study was to establish the influence of some fertile cytoplasm on the carotenoid content in inbred lines and hybrids. Twenty-five isonuclear lines and 100 hybrids were studied for the genetic determinism involved in the transmission of four target carotenoids: lutein, zeaxanthin, β-cryptoxanthin and β-carotene. The analysis of carotenoids was carried out using high performance liquid chromatography using a Flexar system with UV-VIS detection. The obtained data revealed that the cytoplasms did not have a significant influence on the carotenoid content of the inbred lines; larger differences were attributed to the cytoplasm × nucleus interaction. For hybrids, the cytoplasmic nuclear interactions have a significant influence on the content of lutein, zeaxanthin and β-cryptoxanthin. For the cytoplasm × nucleus × tester interactions, significant differences were identified for all traits.
类胡萝卜素是人类饮食中重要的生物活性化合物，因为它们在维持适当的健康状况方面发挥着作用。就产量、单产和收获面积而言，玉米（L.）是全球主要作物之一，它也是全球人类营养中类胡萝卜素的重要来源。提高玉米籽粒中的类胡萝卜素含量是玉米育种研究的主要目标之一；在此背景下，本研究的目的是确定某些可育细胞质对自交系和杂交种中类胡萝卜素含量的影响。对25个同核系和100个杂交种进行了研究，以确定与四种目标类胡萝卜素（叶黄素、玉米黄质、β-隐黄质和β-胡萝卜素）传递相关的遗传决定因素。使用配备紫外-可见检测的Flexar系统通过高效液相色谱法对类胡萝卜素进行分析。获得的数据表明，细胞质对自交系的类胡萝卜素含量没有显著影响；较大的差异归因于细胞质×细胞核相互作用。对于杂交种，细胞质-细胞核相互作用对叶黄素、玉米黄质和β-隐黄质的含量有显著影响。对于细胞质×细胞核×测验种相互作用，所有性状均存在显著差异。
