Jácome-Blásquez Francisco, Ooi Joo Phin, Zeef Leo, Kim Minsung
School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester M13 9PT, UK.
Bioinformatics Core Facility, Faculty of Biology, Medicine and Health, University of Manchester, Manchester M13 9PT, UK.
Plants (Basel). 2022 Jun 22;11(13):1643. doi: 10.3390/plants11131643.
Few species in the genus form plantlets on their leaf margins as an asexual reproduction strategy. The limited molecular studies on plantlet formation show that an organogenesis ortholog, () and embryogenesis genes, such as () and are recruited during plantlet formation. To understand the mechanisms of two plantlet-forming species with different modes of plantlet formation, RNA-sequencing analysis was performed. Differentially expressed genes between the developmental stages were clustered in (Raym.-Hamet and H. Perrier) and (Lam. Pers.), respectively. Of these gene clusters, GO terms that may be involved in plantlet formation of both species, such as signaling, response to wounding, reproduction, regulation of hormone level, and response to karrikin were overrepresented. Compared with the common GO terms, there were more unique GO terms overrepresented during the plantlet formation of each species. A more in-depth investigation is required to understand how these pathways are participating in plantlet formation. Nonetheless, this transcriptome analysis is presented as a reliable basis for future studies on plantlet formation and development in two plantlet-forming species.
该属中很少有物种在叶缘形成小植株作为无性繁殖策略。对小植株形成的有限分子研究表明，在小植株形成过程中会招募一个器官发生直系同源基因（）以及胚胎发生基因，如（）和。为了解两种具有不同小植株形成模式的小植株形成物种的机制，进行了RNA测序分析。发育阶段之间的差异表达基因分别在（雷姆 - 哈梅特和H. 佩里尔）和（林奈 佩尔松）中聚类。在这些基因簇中，可能参与两种物种小植株形成的GO术语，如信号传导、对伤口的反应、繁殖、激素水平调节和对卡里金的反应等，都有过度表达。与常见的GO术语相比，在每个物种的小植株形成过程中，有更多独特的GO术语过度表达。需要更深入的研究来了解这些途径如何参与小植株形成。尽管如此，该转录组分析为未来对两种小植株形成物种的小植株形成和发育研究提供了可靠的基础。
