Gao Jing, Chen Jiyu, Feng Lingyang, Wang Qi, Li Shenglan, Tan Xianming, Yang Feng, Yang Wenyu
College of Agronomy, Sichuan Agricultural University, Huimin Road 211, Wenjiang District, Chengdu 611130, China.
Sichuan Engineering Research Center for Crop Strip Intercropping System, Chengdu 611130, China.
Plants (Basel). 2022 Jun 29;11(13):1728. doi: 10.3390/plants11131728.
Leaf veins constitute the transport network for water and photosynthetic assimilates in vascular plants. The class III homeodomain-leucine zipper () gene family is central to the regulation of vascular development. In this research, we performed an overall analysis of the genes in soybean ( L. Merr.). Our analysis included the phylogeny, conservation domains and -elements in the promoters of these genes. We used the quantitative reverse transcription-polymerase chain reaction to characterize the expression patterns of genes in leaf vein development and analyze the effects of exogenous hormone treatments. In this study, twelve genes were identified from the soybean genome and named. All soybean HD-Zip III proteins contained four highly conserved domains. transcripts showed steadily increasing accumulation during all stages of leaf vein development and were highly expressed in cambium cells. and had nearly identical expression patterns in soybean leaf vein tissues. and transcripts remained at stable high levels at all xylem developmental stages. and were expressed at high levels in the vascular cambium and xylem cells. Overall, may be an essential regulator that is responsible for the formation or maintenance of soybean vein cambial cells. and were correlated with xylem differentiation in soybean leaf veins. This study will pave the way for identifying the molecular mechanism of leaf vein development.
叶脉构成了维管植物中水分和光合产物的运输网络。III类同源异型域-亮氨酸拉链（HD-Zip III）基因家族在维管发育调控中起着核心作用。在本研究中，我们对大豆（Glycine max (L.) Merr.）中的HD-Zip III基因进行了全面分析。我们的分析包括这些基因的系统发育、保守结构域和启动子中的顺式作用元件。我们使用定量逆转录-聚合酶链反应来表征HD-Zip III基因在叶脉发育中的表达模式，并分析外源激素处理的影响。在本研究中，从大豆基因组中鉴定出12个HD-Zip III基因并进行了命名。所有大豆HD-Zip III蛋白都包含四个高度保守的结构域。HD-Zip III转录本在叶脉发育的所有阶段均显示出稳定增加的积累，并在形成层细胞中高表达。GmHB1和GmHB2在大豆叶脉组织中的表达模式几乎相同。GmHB5和GmHB12转录本在木质部发育的所有阶段都保持稳定的高水平。GmHB8和GmHB15在维管形成层和木质部细胞中高表达。总体而言，GmHB8可能是负责大豆叶脉形成层细胞形成或维持的关键调节因子。GmHB5和GmHB12与大豆叶脉中的木质部分化相关。本研究将为阐明叶脉发育的分子机制铺平道路。
