Žitek Taja, Kotnik Petra, Makoter Teo, Postružnik Vesna, Knez Željko, Knez Marevci Maša
Faculty of Chemistry and Chemical Engineering, University of Maribor, Smetanova ul. 17, SI-2000 Maribor, Slovenia.
Faculty of Medicine, University of Maribor, Taborska 8, SI-2000 Maribor, Slovenia.
Plants (Basel). 2022 Jun 30;11(13):1749. doi: 10.3390/plants11131749.
Natural medicines and products are becoming increasingly important in the pharmaceutical and food industries. The most important step in obtaining a natural remedy is the processing of the natural material. This study offers the separation of the industrial hemp plant into fractions by mechanical treatment, which has a significant impact on the selectivity of the obtained fractions. This study also offers a solution to reduce waste by fractionating industrial hemp, focusing on the fraction with the highest cannabinoid content (49.5% of CBD). The study confirmed the anticancer potential of the extract, which prevents further division of WM-266-4 melanoma cells at a concentration of 10 mg/mL. However, application of the extract (c = 10 mg/mL) to normal human epidermal melanocytes proved to be insignificant, as the metabolic activity of the cells was the same as in the control cell group.
天然药物和产品在制药和食品工业中变得越来越重要。获得天然药物的最重要步骤是天然材料的加工。本研究通过机械处理将工业大麻植物分离成不同部分，这对所获得部分的选择性有重大影响。本研究还提供了一种通过将工业大麻分馏来减少浪费的解决方案，重点关注大麻素含量最高的部分（CBD的49.5%）。该研究证实了提取物的抗癌潜力，其在浓度为10mg/mL时可阻止WM-266-4黑色素瘤细胞的进一步分裂。然而，将提取物（c = 10mg/mL）应用于正常人表皮黑素细胞被证明是无显著影响的，因为细胞的代谢活性与对照细胞组相同。
