Chesselet M F, Affolter H U
Brain Res. 1987 Apr 28;410(1):83-8. doi: 10.1016/s0006-8993(87)80024-0.
Sections from postmortem human brain were processed for in situ hybridization histochemistry using a 35S-labelled RNA probe transcribed from a cDNA coding for the human preprotachykinin which contains both substance P and K. Labelled neurons were observed in the caudate nucleus and the putamen but not the cerebellum. The labelled cells were of medium size and their distribution and morphology were compatible with previous data on substance P-like immunoreactivity in the human brain. The results confirm the presence of preprotachykinin mRNA in a subpopulation of striatal neurons in the human and show that in situ hybridization can be used to detect specific neurotransmitter-related mRNAs in postmortem tissue from normal and diseased humans.
利用从编码人前速激肽原的cDNA转录而来的35S标记RNA探针，对人尸检脑切片进行原位杂交组织化学处理。该前速激肽原同时包含P物质和神经激肽K。在尾状核和壳核中观察到标记神经元，但在小脑中未观察到。标记细胞为中等大小，其分布和形态与前人关于人脑中P物质样免疫反应性的数据相符。结果证实人纹状体神经元亚群中存在前速激肽原mRNA，并表明原位杂交可用于检测正常人和患病者尸检组织中特定神经递质相关的mRNA。
