Centre for Biomedical Technologies, School of Mechanical, Medical and Process Engineering, Queensland University of Technology, 60 Musk Ave/cnr. Blamey St, Kelvin Grove, Brisbane, QLD, 4059, Australia.
Central Analytical Research Facility, Queensland University of Technology, Brisbane, 4059, Australia.
Histochem Cell Biol. 2022 Nov;158(5):463-469. doi: 10.1007/s00418-022-02135-8. Epub 2022 Jul 9.
The anatomy of the osteochondral junction is complex because several tissue components exist as a unit, including uncalcified cartilage (with superficial, middle, and deep layers), calcified cartilage, and subchondral bone. Furthermore, it is difficult to study because this region is made up of a variety of cell types and extracellular matrix compositions. Using X-ray fluorescence microscopy, we present a protocol for simultaneous elemental detection on fresh frozen samples. We transferred the osteochondral sample using a tape-assisted system and successfully tested it in synchrotron X-ray fluorescence. This protocol elucidates the distinct distribution of elements at the human knee's osteochondral junction, making it a useful tool for analyzing the co-distribution of various elements in both healthy and diseased states.
骨软骨连接的解剖结构很复杂，因为有几个组织成分作为一个单元存在，包括未钙化软骨（包括表层、中层和深层）、钙化软骨和软骨下骨。此外，由于该区域由多种细胞类型和细胞外基质组成，因此很难进行研究。我们使用 X 射线荧光显微镜，提出了一种对新鲜冷冻样本进行同时元素检测的方案。我们使用胶带辅助系统转移骨软骨样本，并在同步加速器 X 射线荧光中成功地对其进行了测试。该方案阐明了人膝关节骨软骨连接处元素的独特分布，使其成为分析健康和患病状态下各种元素共同分布的有用工具。
