Fan Xiwei, Donose Bogdan, Jones Michael W M, Howard Daryl, Torniainen Jari, Bertling Karl, Guo Xiao, Kewish Cameron M, Lee Kah Meng, Sun Antonia Rujia, Rakic Aleksandar, Crawford Ross, Afara Isaac O, Prasadam Indira
Department of Orthopaedic Surgery, The Second Xiangya Hospital of Central South University, Changsha, China.
School of Mechanical, Medical & Process Engineering, Queensland University of Technology, 60 Musk Ave/Cnr. Blamey St, Kelvin Grove, Brisbane, QLD, 4059, Australia.
Histochem Cell Biol. 2024 Dec 2;163(1):16. doi: 10.1007/s00418-024-02338-1.
Understanding the osteochondral junction, where non-mineralised cartilage and mineralised bone converge, is crucial for joint health. Current sample preparation techniques are insufficient for detailed spatial hyperspectral imaging analysis. Using the enhanced Kawamoto method, we used the super cryo embedding medium's temperature-dependent properties to transfer high-quality tissue samples onto slides for spatial imaging analysis. We transferred osteochondral samples using a tape-free system and successfully tested them in hematoxylin and eosin (HE), Safranin-O, nanomechanical assessments and nano-Fourier transform infrared (FTIR) mapping. This protocol elucidates the structural and elemental gradients, mechanical characteristics and distinctive biochemical layering, making it a useful tool for analysing biochemical properties' co-distribution in healthy and diseased situations.
了解非矿化软骨和矿化骨交汇的骨软骨交界处对于关节健康至关重要。目前的样本制备技术不足以进行详细的空间高光谱成像分析。我们采用改良的川本方法，利用超低温包埋介质的温度依赖性特性，将高质量的组织样本转移到载玻片上进行空间成像分析。我们使用无胶带系统转移骨软骨样本，并成功地在苏木精和伊红（HE）染色、番红O染色、纳米力学评估和纳米傅里叶变换红外（FTIR）映射中对其进行了测试。该方案阐明了结构和元素梯度、力学特性以及独特的生化分层，使其成为分析健康和疾病状态下生化特性共分布的有用工具。
