水浸贯众提取物经口服摄入后可刺激人尿液中 Tamm-Horsfall 蛋白的分泌。
Aqueous extract from Equisetum arvense stimulates the secretion of Tamm-Horsfall protein in human urine after oral intake.
机构信息
Institute of Pharmaceutical Biology and Phytochemistry, University of Münster, Corrensstraße 48, Münster D-48149, Germany.
MEET - Münster Electrochemical Energy Technology, University of Münster, Corrensstraße 48, Münster D-48149, Germany.
出版信息
Phytomedicine. 2022 Sep;104:154302. doi: 10.1016/j.phymed.2022.154302. Epub 2022 Jul 1.
BACKGROUND
Within European traditional phytotherapy, extracts from different herbal plants are used for prevention and therapy of uncomplicated urinary tract infections and for flushing out of kidney grits. Besides increased urine flow by slight diuretic effects, also stimulation of Tamm-Horsfall protein (syn. THP, uromodulin) in the distal part of the kidney could explain reduced kidney gravel and anti-virulent activity against uropathogenic E. coli.
PURPOSES
Evaluation of THP-inducing activity of extracts from Equisetum arvense, Levisticum officinalis, Ilex paraguariensis, Juniperus communis, Urtica dioica, and Taraxacum officinale by quantification of THP in urine samples after oral application to humans.
STUDY DESIGN
7 days p.o. application of the test intervention to healthy volunteers (n = 10 per intervention group) and analysis of urine samples at day 1 (untreated control values), and days 3, 6 and 8 on THP content by validated ELISA. Antiadhesive activity of urine samples was monitored by flow cytometry using UPEC strain NU14 against human T24 bladder cells.
RESULTS
An aqueous extract from E. arvense, fully characterized by a specific LC-MS method, induced THP concentration in urine samples significantly during a 7-day p.o. application up to 300%, related to the untreated controls. Ex vivo investigation of the individual and pooled urine samples with elevated THP concentrations showed good correlation to antiadhesive effects against UPEC NU14 to T24 cells. Urine samples of the Equisetum treated volunteers had no effect on the proliferation and on biofilm formation of UPEC NU14. Silica excretion in the urine samples had no correlation to the respective THP levels. Monitoring of electrolyte content in the urine samples indicat ed diuretic effects of the intervention with Equisetum extract. Detailed phytochemical analysis of the Equisetum extract by LC-MS and LC-UV revealed an analytical protocol, which identified > 80 compounds from the extract by MS evaluations and 18 compounds by UV detection. This protocol will provide a valuable tool for future quality control of Equisetum extract.
CONCLUSION
Aqueous extract from E. arvense significantly stimulates THP secretion in urine samples after 7 days of oral intake and inhibits the interplay between UPEC and bladder host cells. This could explain the therapeutic use of this herbal material for urinary tract infections and kidney gravel. Detailed phytochemical analysis of the Equisetum extract by LC-MS and LC-UV revealed an analytical protocol, which identified > 82% of all eluted compounds. This protocol will provide a valuable tool for future quality control of Equisetum extract.
背景
在欧洲传统植物疗法中，从不同草药植物中提取的物质被用于预防和治疗单纯性尿路感染，并用于冲洗肾脏结石。除了通过轻微的利尿作用增加尿液流量外，在肾脏的远端部分刺激 Tamm-Horsfall 蛋白（也称为 THP，尿调蛋白）也可以解释减少肾结石和对尿路致病性大肠杆菌的抗毒活性。
目的
通过定量分析口服给予人体后尿液样本中的 THP，评估水飞蓟素、独活、贯叶连翘、杜松、荨麻和蒲公英提取物对 Tamm-Horsfall 蛋白（THP）的诱导活性。
研究设计
7 天口服给予健康志愿者（每组 10 名志愿者）试验干预措施，并通过验证的 ELISA 分析尿液样本中 THP 的含量，在第 1 天（未处理的对照值）和第 3、6 和 8 天。使用 UPEC 菌株 NU14 对抗人 T24 膀胱细胞，通过流式细胞术监测尿液样本的抗粘附活性。
结果
一种通过特定的 LC-MS 方法充分表征的水飞蓟素提取物，在 7 天口服给药期间，使尿液样本中的 THP 浓度显著增加，最高可达未处理对照组的 300%。对具有升高 THP 浓度的个体和混合尿液样本的离体研究显示，与 UPEC NU14 对 T24 细胞的抗粘附作用具有良好的相关性。来自服用水飞蓟素的志愿者的尿液样本对 UPEC NU14 的增殖和生物膜形成没有影响。尿液样本中的硅排泄与各自的 THP 水平没有相关性。尿液样本中电解质含量的监测表明，水飞蓟素提取物干预具有利尿作用。通过 LC-MS 和 LC-UV 对水飞蓟素提取物进行的详细植物化学分析揭示了一种分析方案，通过 MS 评估鉴定了提取物中的>80 种化合物，通过 UV 检测鉴定了 18 种化合物。该方案将为水飞蓟素提取物的未来质量控制提供有价值的工具。
结论
口服摄入 7 天后，水飞蓟素提取物可显著刺激尿液样本中 THP 的分泌，并抑制 UPEC 和膀胱宿主细胞之间的相互作用。这可以解释这种草药材料在尿路感染和肾结石方面的治疗用途。通过 LC-MS 和 LC-UV 对水飞蓟素提取物进行的详细植物化学分析揭示了一种分析方案，通过 MS 评估鉴定了提取物中的>82%的洗脱化合物。该方案将为水飞蓟素提取物的未来质量控制提供有价值的工具。
Zotero 插件