Vadeboncoeur C, Gauthier L
Can J Microbiol. 1987 Feb;33(2):118-22. doi: 10.1139/m87-020.
A double-spontaneous mutant resistant to the growth inhibitory effect of alpha-methylglucoside and 2-deoxyglucose was isolated from Streptococcus salivarius. This mutant strain, called alpha S3L11, did not grow on mannose and grew poorly on 5 mM fructose and 5 mM glucose. Isolated membranes of strain alpha S3L11 were unable to catalyse the phosphoenolpyruvate-dependent phosphorylation of mannose in the presence of purified enzyme I and HPr. Addition of dialysed membrane-free cellular extract of the wild-type strain to the reaction medium restored the activity. The factor that restored the phosphoenolpyruvate-mannose phosphotransferase activity to membranes of strain alpha S3L11 was called IIIman. This factor was partially purified from the wild-type strain by DEAE-cellulose chromatography, DEAE-TSK chromatography, and molecular seiving on a column of Ultrogel AcA 34. This partially purified preparation also enhanced the phosphoenolpyruvate-dependent phosphorylation of glucose, fructose, and 2-deoxyglucose in strain alpha S3L11.
从唾液链球菌中分离出一株对α-甲基葡萄糖苷和2-脱氧葡萄糖的生长抑制作用具有抗性的双自发突变体。这个突变菌株称为αS3L11，它不能在甘露糖上生长，在5 mM果糖和5 mM葡萄糖上生长较差。在纯化的酶I和HPr存在的情况下，αS3L11菌株的分离膜无法催化磷酸烯醇丙酮酸依赖性的甘露糖磷酸化。向反应培养基中添加野生型菌株的透析后的无膜细胞提取物可恢复活性。将恢复αS3L11菌株膜的磷酸烯醇丙酮酸-甘露糖磷酸转移酶活性的因子称为IIIman。通过DEAE-纤维素色谱法、DEAE-TSK色谱法以及在Ultrogel AcA 34柱上进行分子筛分，从野生型菌株中对该因子进行了部分纯化。这种部分纯化的制剂还增强了αS3L11菌株中磷酸烯醇丙酮酸依赖性的葡萄糖、果糖和2-脱氧葡萄糖的磷酸化作用。
