Integrated Bioprocessing Laboratory, Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology (SRM IST), Kattankulathur, 603 203, India.
Department of Chemical Engineering, Sri Sivasubramaniya Nadar College of Engineering, Chennai, 603110, Tamilnadu, India; Centre of Excellence in Water Research (CEWAR), Sri Sivasubramaniya Nadar College of Engineering, Chennai, 603 110, Tamilnadu, India; Department of Biotechnology Engineering and Food Technology, Chandigarh University, Mohali, 140413, India.
Environ Pollut. 2022 Sep 15;309:119729. doi: 10.1016/j.envpol.2022.119729. Epub 2022 Jul 6.
The treatment of contaminants from lignocellulosic biorefinery effluent has recently been identified as a unique challenge. This study focuses on removing phenolic contaminants and polycyclic aromatic hydrocarbons (PAHs) from lignocellulosic biorefinery wastewater (BRW) applying a laccase-assisted approach. Cassava waste was used as a substrate to produce the maximum yield of laccase enzyme (3.9 U/g) from Pleurotus ostreatus. Among the different inducers supplemented, CuSO (0.5 mM) showed an eight-fold increase in enzyme production (30.8 U/g) after 240 h of incubation. The catalytic efficiency of laccase was observed as 128.7 ± 8.47 SmM for syringaldazine oxidation at optimum pH 4.0 and 40 °C. Laccase activity was completely inhibited by lead (II) ion, mercury (II) ion, sodium dodecyl sulphate, sodium azide and 1,4 dithiothretiol and induced significantly by manganese (II) ion and rhamnolipid. After treating BRW with laccase, the concentrations of PAHs and phenolic contaminants of 1144 μg/L and 46160 μg/L were reduced to 96 μg/L and 16100 μg/L, respectively. The ability of laccase to effectively degrade PAHs in the presence of different phenolic compounds implies that phenolic contaminants may play a role in PAHs degradation. After 240 h, organic contaminants were removed from BRW in the following order: phenol >2,4-dinitrophenol > 2-methyl-4,6-dinitrophenol > 2,3,4,6-tetrachlorophenol > acenaphthene > fluorine > phenanthrene > fluoranthene > pyrene > anthracene > chrysene > naphthalene > benzo(a)anthracene > benzo(a)pyrene > benzo(b)fluoranthene > pentachlorophenol > indeno(1,2,3-cd)pyrene > benzo(j) fluoranthene > benzo[k]fluoranthène. The multiple contaminant remediation from the BRW by enzymatic method, clearly suggests that the laccase can be used as a bioremediation tool for the treatment of wastewater from various industries.
木质纤维素生物炼制厂废水的处理最近被认为是一个独特的挑战。本研究采用漆酶辅助方法，从木质纤维素生物炼制厂废水中去除酚类污染物和多环芳烃（PAHs）。木薯废料被用作基质，从糙皮侧耳（Pleurotus ostreatus）中生产出最大产量的漆酶酶（3.9 U/g）。在补充的不同诱导物中，CuSO（0.5 mM）在孵育 240 小时后使酶产量增加了八倍（30.8 U/g）。漆酶在最佳 pH 值 4.0 和 40°C 下对愈创木酚氧化的催化效率为 128.7±8.47 SmM。铅（II）离子、汞（II）离子、十二烷基硫酸钠、叠氮化钠和 1,4 二硫苏糖醇完全抑制了漆酶的活性，而锰（II）离子和鼠李糖脂则显著诱导了漆酶的活性。用漆酶处理 BRW 后，将 1144μg/L 的多环芳烃和 46160μg/L 的酚类污染物浓度分别降低至 96μg/L 和 16100μg/L。漆酶在存在不同酚类化合物的情况下有效降解多环芳烃的能力表明，酚类污染物可能在多环芳烃降解中起作用。240 小时后，BRW 中的有机污染物按以下顺序去除：苯酚>2,4-二硝基苯酚>2-甲基-4,6-二硝基苯酚>2,3,4,6-四氯苯酚>苊>氟>菲>荧蒽>芘>蒽>荧蒽>萘>苯并（a）蒽>苯并（a）芘>苯并（b）荧蒽>五氯苯酚>茚并（1,2,3-cd）芘>苯并（j）荧蒽>苯并（k）荧蒽。通过酶法从 BRW 中去除多种污染物，清楚地表明漆酶可用作处理各种工业废水的生物修复工具。
