Dong Haiwen, Liu Wei, Zhang Hao, Zheng Xuebo, Duan Huijie, Zhou Lixiu, Xu Tongtong, Ruan Roger
Qilu University of Technology (Shandong Academy of Sciences), Shandong Analysis and Test Center, Jinan, Shandong, 250014, China.
Qilu University of Technology (Shandong Academy of Sciences), Shandong Analysis and Test Center, Jinan, Shandong, 250014, China.
Chemosphere. 2022 Nov;306:135604. doi: 10.1016/j.chemosphere.2022.135604. Epub 2022 Jul 6.
A symbiotic system of algae-bacteria, and attached cultivation, are two ways to increase microalgae biomass, and beneficially effect wastewater treatment. However, the possible advantages of the algae-bacteria co-culture in attached cultivation, are still unclear. This paper investigates the effects of different morphologies of a phosphate solubilizing bacteria-Paenibacillus xylanexedens (bacteria supernatant, bacteria, broken bacteria), on the growth of microalgae-Chlorella pyrenoidosa and wastewater treatment in an attached co-culture system. The results show that the broken bacteria had the most significant effect, with the biomass and protein content of Chlorella pyrenoidosa increasing by 125.67% and 25.04%; and the removal rate of COD, NH-N and PO in wastewater increasing by 23.57%, 146.15% and 9.96% respectively. This indicates that the intracellular material of the Paenibacillus xylanexedens was more effective in promoting the biomass growth of Chlorella pyrenoidosa and the removal rates of COD, NH-N and PO, compared to the algae growing without the bacteria. The algae-bacteria symbiotic attached mode was superior to the suspended mode, in terms of both Chlorella pyrenoidosa biomass enhancement and effective wastewater treatment. The addition of different morphologies of Paenibacillus xylanexedens significantly enlarged the difference between the two culture modes. This study provides a new method for coupled algae-bacteria co-cultures for wastewater treatment, based on the symbiotic effect.
藻菌共生系统和附着培养是增加微藻生物量并对废水处理产生有益影响的两种方式。然而，附着培养中藻菌共培养的潜在优势仍不明确。本文研究了不同形态的解磷细菌——木聚糖栖芽孢杆菌（细菌上清液、完整细菌、破碎细菌）对附着共培养系统中微藻——蛋白核小球藻生长及废水处理的影响。结果表明，破碎细菌的影响最为显著，蛋白核小球藻的生物量和蛋白质含量分别增加了125.67%和25.04%；废水中化学需氧量、氨氮和磷酸盐的去除率分别提高了23.57%、146.15%和9.96%。这表明，与无细菌生长的藻类相比，木聚糖栖芽孢杆菌的细胞内物质在促进蛋白核小球藻生物量增长以及化学需氧量、氨氮和磷酸盐的去除率方面更有效。在蛋白核小球藻生物量增加和有效废水处理方面，藻菌共生附着模式均优于悬浮模式。添加不同形态的木聚糖栖芽孢杆菌显著扩大了两种培养模式之间的差异。本研究基于共生效应，为废水处理的藻菌联合共培养提供了一种新方法。
