Dong Haiwen, Liu Wei, Zhang Hao, Wang Zhenhua, Feng Fei, Zhou Lixiu, Duan Huijie, Xu Tongtong, Li Xiaomeng, Ma Junjian
Qilu University of Technology (Shandong Academy of Sciences), Shandong Analysis and Test Center, Jinan, 250014, Shandong, China.
Faculty of Environmental Science and Technology, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250353, Shandong, China.
Bioprocess Biosyst Eng. 2023 May;46(5):707-716. doi: 10.1007/s00449-023-02855-8. Epub 2023 Feb 24.
Algae-bacteria symbiosis can promote the growth of microalgae and improve the efficiency of wastewater treatment. Attached culture is an efficient culture technique for microalgae, with benefits of high yield, low water consumption and easy harvesting. However, the promoting effects of bacteria on microalgae in attached culture are still unclear. In this study, different forms of a nitrogen-fixing bacteria, Azotobacter beijerinckii (including bacteria supernatant, live bacteria, and broken bacteria), were co-cultured with Chlorella pyrenoidosa in an attached culture system using wastewater as the culture medium. The results showed that the broken A. beijerinckii form had the best growth promotion effect on C. pyrenoidosa. Compared with the pure algae culture, the biomass of C. pyrenoidosa increased by 71.8% and the protein increased by 28.2%. The live bacteria form had the best effect on improving the efficiency of wastewater treatment by C. pyrenoidosa, with the COD, PO and NH-N removal rates increased by 20.8%, 18.5% and 8.9%, respectively, in comparison with the pure algae culture. The attached co-culture mode promoted the growth of C. pyrenodisa better than the suspended co-culture mode. This research offers a new way for improving microalgae biomass and wastewater treatment by attached algae-bacteria symbiont.
藻菌共生可促进微藻生长并提高废水处理效率。附着培养是一种高效的微藻培养技术，具有产量高、耗水量低和易于收获等优点。然而，附着培养中细菌对微藻的促进作用仍不明确。在本研究中，以固氮菌拜氏固氮菌的不同形式（包括细菌上清液、活细菌和破碎细菌）与小球藻在以废水为培养基的附着培养系统中进行共培养。结果表明，破碎的拜氏固氮菌形式对小球藻的生长促进效果最佳。与纯藻培养相比，小球藻的生物量增加了71.8%，蛋白质增加了28.2%。活细菌形式对小球藻提高废水处理效率的效果最佳，与纯藻培养相比，化学需氧量、总磷和氨氮的去除率分别提高了20.8%、18.5%和8.9%。附着共培养模式比悬浮共培养模式更能促进小球藻的生长。本研究为通过附着藻菌共生体提高微藻生物量和废水处理提供了一种新途径。
