Department of ICU, The First Hospital of Jilin University, Changchun 130021, PR China.
Department of Neonatology, The First Hospital of Jilin University, Changchun 130021, PR China.
Genomics. 2022 Jul;114(4):110428. doi: 10.1016/j.ygeno.2022.110428. Epub 2022 Jul 6.
BACKGROUND: Long noncoding RNAs (lncRNAs) can mediate the biological processes during tumorigenesis which may be affected by tumor associated macrophages (TAMs). Hence, we aim to identify the functionality of LINC00702 in regulation of bladder cancer cells and M2-TAMs. METHODS: After induction of M2-TAMs from THP-1 monocyte, we evaluated effects of LINC00702 on bladder cancer cells and M2-TAMs, which were validated in a xenograft tumor mouse model. RESULTS: Low LINC00702 expression was determined in bladder cancer tissues. LINC00702 could promote DUSP1 transcription by recruiting JUND to its promoter. Ectopic LINC00702 expression suppressed the bladder cancer cell proliferation and secretion of inflammatory cytokines by M2-TAMs through up-regulation of DUSP1. The anti-tumor activity of LINC00702 was ultimately validated in vivo. CONCLUSION: LINC00702 promoted DUSP1 by recruiting JUND to inhibit the proliferation of bladder cancer cells and the secretion of inflammatory factors, thus modulating bladder cancer inflammatory microenvironment.
背景：长链非编码 RNA（lncRNA）可在肿瘤发生过程中介导生物学过程，这些过程可能受肿瘤相关巨噬细胞（TAMs）影响。因此，我们旨在鉴定 LINC00702 在调控膀胱癌细胞和 M2-TAMs 中的功能。
方法：用 THP-1 单核细胞诱导 M2-TAMs 后，我们评估了 LINC00702 对膀胱癌细胞和 M2-TAMs 的影响，并在异种移植肿瘤小鼠模型中进行了验证。
结果：LINC00702 在膀胱癌组织中表达水平较低。LINC00702 通过招募 JUND 到其启动子上来促进 DUSP1 的转录。过表达 LINC00702 通过上调 DUSP1 抑制 M2-TAMs 促进的膀胱癌细胞增殖和炎症因子的分泌。LINC00702 的抗肿瘤活性最终在体内得到验证。
结论：LINC00702 通过招募 JUND 促进 DUSP1，从而抑制膀胱癌细胞的增殖和炎症因子的分泌，从而调节膀胱癌炎症微环境。
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