Eisbruch A, Blick M, Lee J S, Sacks P G, Gutterman J
Cancer Res. 1987 Jul 1;47(13):3603-5.
Seventeen fresh uncultured tumors obtained from biopsies of patients with various forms of head and neck cancer and two squamous cell carcinoma cell lines were analyzed for RNA expression and structural alterations of the epidermal growth factor reception gene. We used cDNA probes for the external and the internal domains of the gene. Enhanced mRNA expression was found only in one squamous cell carcinoma cell line, which is known to have high levels of epidermal growth factor receptor. No amplification or structural rearrangement of the epidermal growth factor receptor gene was found.
对从患有各种头颈癌的患者活检中获取的17个新鲜未培养肿瘤以及两个鳞状细胞癌细胞系进行了分析，以检测表皮生长因子受体基因的RNA表达和结构改变。我们使用了针对该基因外部和内部结构域的cDNA探针。仅在一个已知具有高水平表皮生长因子受体的鳞状细胞癌细胞系中发现了增强的mRNA表达。未发现表皮生长因子受体基因的扩增或结构重排。
