Key Laboratory of Straw Comprehensive Utilization and Black Soil Conservation, The Ministry of Education, College of Life Science, Jilin Agricultural University, Changchun, 130118, Jilin, China; Straw Biological Conversion and Agricultural Utilization Engineering Research Center of Jilin Province, China.
Key Laboratory of Straw Comprehensive Utilization and Black Soil Conservation, The Ministry of Education, College of Life Science, Jilin Agricultural University, Changchun, 130118, Jilin, China.
Environ Pollut. 2022 Oct 1;310:119726. doi: 10.1016/j.envpol.2022.119726. Epub 2022 Jul 7.
It is estimated that over 700,000 tons of synthetic dyes are produced annually, 15% of which are emitted as effluents. These highly stable dyes enter the world water ecosystems and stay in the environment, and eventually cause adverse impacts to the environment. Current wastewater treatment methods, such as filtration, coagulation, and chemical oxidation, have sideeffects, including toxic residue formation, membrane fouling, bioaccumulation, and secondary pollutant formation. Given the issues mentioned, it is necessary to study how to improve the degradation of synthetic dye with a cost-effective and ecofriendly approach. Natural oxidation provides a greener option. Recently, Deuteromycetes fungus Myrothecium verrucaria G-1 (M. verrucaria G-1) has shown great potential in producing high level of dye oxidase. This study aims to generate a dye oxidase hyperproducer, 3H6 from M. verrucaria G-1 by using atmospheric and room temperature plasma (ARTP) coupled with ultraviolet (UV) irradiation. This method increases oxidase production by nearly 106.15%. After a simple precipitation and dialysis, this mutant oxidase increases by 1.97-fold in a specific activity with dye degradation rates at 70% for Mmethylene blue (MB) and 85% for Congo red (CR). It is found that the genetic stability of 3H6 remains active for ten generations. The size of oxidase is 65 kDa, and optimum temperature for reaction is 30 °C with 4.5 pH. This study presents that the first combined mutagenesis approach by ARPT-UV on fungus species generates an impressive increment of acid dye oxidases production. As such, this method presents a cost-effective alternative to mitigate hazardous dye pollution.
据估计，每年生产超过 70 万吨合成染料，其中 15%作为废水排放。这些高度稳定的染料进入世界水生态系统并留在环境中，最终对环境造成不利影响。目前的废水处理方法，如过滤、混凝和化学氧化，都有副作用，包括有毒残留物的形成、膜污染、生物积累和二次污染物的形成。鉴于上述问题，有必要研究如何用一种具有成本效益和环保的方法来提高合成染料的降解效率。自然氧化提供了一种更环保的选择。最近，被孢霉属真菌 M. verrucaria G-1（M. verrucaria G-1）在产生高水平染料氧化酶方面显示出巨大的潜力。本研究旨在利用大气压室温等离子体（ARTP）与紫外线（UV）辐照相结合，从 M. verrucaria G-1 中生成染料氧化酶高产菌 3H6。该方法使氧化酶产量增加了近 106.15%。经过简单的沉淀和透析，该突变体氧化酶的比活性提高了 1.97 倍，对亚甲基蓝（MB）的降解率达到 70%，对刚果红（CR）的降解率达到 85%。研究发现，3H6 的遗传稳定性在十代内仍然保持活性。氧化酶的大小为 65 kDa，反应的最适温度为 30°C，pH 值为 4.5。本研究首次提出，ARPT-UV 联合诱变真菌可显著提高酸性染料氧化酶的产量。因此，这种方法为减轻危险染料污染提供了一种具有成本效益的替代方案。
