Hebei Key Laboratory of Quality & Safety Analysis-Testing for Agro-Products and Food, Hebei North University, Zhangjiakou City, Hebei Province, China 075000.
College of Agriculture and Forestry Science and Technology, North University of Hebei, Zhangjiakou City, Hebei Province, China 075000.
Comput Math Methods Med. 2022 Jun 29;2022:7134161. doi: 10.1155/2022/7134161. eCollection 2022.
In order to study the problem that grapes are vulnerable to microbial infection and decay during storage, a method based on antagonistic Burkholderia contaminans against postharvest Botrytis cinerea of Rosa vinifera was proposed in this paper. The method tested the resistance induction mechanism of Botrytis cinerea after harvest and determined the fruit decay rate treated by antagonistic Burkholderia contaminans. The results showed that the antagonistic bacterium B-1 had bacteriostatic effect on many common pathogens of fruits and vegetables to a certain extent, and the bacteriostatic range was wide. Among them, the inhibition rate of Fusarium moniliforme was 75.5% and that of Botrytis cinerea was 51.2%. After testing, it can be found that antagonistic bacteria have an inhibitory effect on pathogenic fungi and have an effect on phenylpropane metabolic pathway, reactive oxygen species metabolic pathway, and the activities of other resistance-related enzymes. Through comparison, it can be found that the antagonistic Burkholderia contaminans has a strong antibacterial mechanism against Botrytis cinerea of rose grape after harvest. The fruit treated with antagonistic B Burkholderia B-1 has significantly reduced the decay rate and increased the activity of antibacterial active protein.
为了研究葡萄在贮藏过程中易受微生物感染和腐烂的问题,本文提出了一种基于拮抗菌丛枝菌根伯克霍尔德氏菌防治蔷薇科葡萄采后灰霉病的方法。该方法检测了采后灰霉病的抗性诱导机制,并测定了拮抗菌丛枝菌根伯克霍尔德氏菌处理后的果实腐烂率。结果表明,拮抗菌 B-1 对多种常见果蔬病原菌具有一定的抑菌作用,抑菌范围较宽。其中,对串珠镰刀菌的抑制率为 75.5%,对灰霉病的抑制率为 51.2%。经过测试,可以发现拮抗菌对病原真菌有抑制作用,对苯丙烷代谢途径、活性氧代谢途径和其他抗性相关酶的活性都有影响。通过比较可以发现,拮抗菌丛枝菌根伯克霍尔德氏菌对蔷薇科葡萄采后灰霉病具有较强的抑菌机制。用拮抗菌 B-1 处理的果实腐烂率显著降低,抗菌活性蛋白的活性增加。