Ye Ziyu, Liang Yanfang, Lin Bihua, Li Yanyun, Chai Xingxing, Lian Jiachun, Zhang Xueying, Che Zhengping, Zeng Jincheng
Dongguan Key Laboratory of Medical Bioactive Molecular Developmental and Translational Research, Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Guangdong Medical University, Dongguan 523808, China.
Guangdong Xinghai Institute of Cell, Dongguan 523808, China.
Stem Cells Int. 2022 Jun 30;2022:6544514. doi: 10.1155/2022/6544514. eCollection 2022.
Human gingiva-derived mesenchymal stem cells (GMSCs) are isolated from the gingival propria with promising regenerative, immunomodulatory, and anti-inflammatory properties. Recently, several studies, including ours, have found that GMSCs have the therapeutic potentials of nerve regeneration and skin disorders in various types such as the cell itself, cell-free conditioned medium, or extracellular vesicles (EVs). However, the mechanobiological behavior of GMSCs is closely related to the culture conditions. Therefore, the purpose of this study was to evaluate the function of human GMSCs on imiquimod- (IMQ-) induced murine psoriasis-like skin inflammation in two-dimensional (2D) and three-dimensional (3D) culture conditions. Here, we isolated and characterized GMSCs in 2D and 3D culture conditions and found that GMSCs in 2D and 3D infusion can significantly ameliorate the IMQ-induced murine psoriasis-like skin inflammation, reduce the levels of Th1- and Th17-related cytokines IFN-, TNF-, IL-6, IL-17A, IL-17F, IL-21, and IL-22, and upregulate the percentage of spleen CD25CD3 T cells while downregulate the percentage of spleen IL-17CD3 T cells. In summary, our novel findings reveal that GMSCs in 2D and 3D infusion may possess therapeutic effects in the treatment of psoriasis.
人牙龈间充质干细胞（GMSCs）是从牙龈固有层分离出来的，具有再生、免疫调节和抗炎等特性。最近，包括我们的研究在内的多项研究发现，GMSCs在治疗神经再生和各种类型的皮肤疾病方面具有治疗潜力，如细胞本身、无细胞条件培养基或细胞外囊泡（EVs）。然而，GMSCs的机械生物学行为与培养条件密切相关。因此，本研究的目的是评估在二维（2D）和三维（3D）培养条件下，人GMSCs对咪喹莫特（IMQ）诱导的小鼠银屑病样皮肤炎症的作用。在此，我们在2D和3D培养条件下分离并鉴定了GMSCs，发现2D和3D注入的GMSCs可显著改善IMQ诱导的小鼠银屑病样皮肤炎症，降低Th1和Th17相关细胞因子IFN-、TNF-、IL-6、IL-17A、IL-17F、IL-21和IL-22的水平，并上调脾脏CD25⁺CD3⁺ T细胞的百分比，同时下调脾脏IL-17⁺CD3⁺ T细胞的百分比。总之，我们的新发现揭示了2D和3D注入的GMSCs在治疗银屑病方面可能具有治疗作用。
