Goldsmith B M, Feinstein C, Munson S, Reiss A, Borengasser-Caruso M A
Clin Biochem. 1986 Dec;19(6):359-63. doi: 10.1016/s0009-9120(86)80009-1.
Whole blood serotonin (5HT) concentrations were measured in a group of children and adolescents to determine a reference range for this population. Blood was collected after at least a 6-hour fast, mixed with ascorbic acid and EDTA, and frozen at -70 degrees C until analysis. 5HT was determined by HPLC with electrochemical detection. Matrix-matched whole blood standards and controls were used to determine 5HT concentrations, and monitor performance of the assay. 5HT concentrations in boys ranged from 0.53 to 3.13 mumol/L (mean = 1.27, SD = 0.47) while the range for girls was 0.63 to 2.46 mumol/L (mean = 1.21, SD = 0.47). There was no significant difference in 5HT concentrations between boys and girls, nor was there any significant change in 5HT concentration with age. The nonparametric central 95 percent reference range for boys and girls was determined to be 0.64-2.45 mumol/L.
对一组儿童和青少年测量全血血清素（5-羟色胺，5HT）浓度，以确定该人群的参考范围。在至少禁食6小时后采集血液，与抗坏血酸和乙二胺四乙酸（EDTA）混合，并在-70℃下冷冻直至分析。采用高效液相色谱法（HPLC）和电化学检测法测定5HT。使用基质匹配的全血标准品和对照品来确定5HT浓度，并监测检测方法的性能。男孩的5HT浓度范围为0.53至3.13μmol/L（平均值 = 1.27，标准差 = 0.47），而女孩的范围为0.63至2.46μmol/L（平均值 = 1.21，标准差 = 0.47）。男孩和女孩的5HT浓度没有显著差异，5HT浓度也没有随年龄的显著变化。男孩和女孩的非参数中心95%参考范围确定为0.64 - 2.45μmol/L。
