Keating J, Dratcu L, Lader M, Sherwood R A
Department of Clinical Biochemistry, Kings College School of Medicine and Dentistry, Denmark Hill, London, UK.
J Chromatogr. 1993 Jun 2;615(2):237-42. doi: 10.1016/0378-4347(93)80337-4.
A reversed-phase high-performance liquid chromatographic method is described for the measurement of plasma serotonin concentrations. Sample preparation is by a simple solid-phase extraction using C18 columns. An isocratic separation is used with electrochemical detection. The application of the method to the measurement of plasma serotonin concentrations following the administration of fluvoxamine (a serotonin re-uptake inhibitor), maprotiline (a noradrenaline re-uptake inhibitor) and placebo to normal subjects for a seven-day period is reported. Fluvoxamine significantly decreases plasma serotonin over this time period in a linear fashion. No effect on plasma serotonin was seen for maprotiline or placebo. Plasma serotonin concentrations can be used to monitor compliance with fluvoxamine therapy.
本文描述了一种反相高效液相色谱法，用于测定血浆中血清素的浓度。样品制备采用简单的C18柱固相萃取法。采用等度洗脱分离并结合电化学检测。报道了该方法在正常受试者连续七天服用氟伏沙明（一种血清素再摄取抑制剂）、马普替林（一种去甲肾上腺素再摄取抑制剂）和安慰剂后，用于测定血浆血清素浓度的应用情况。在此时间段内，氟伏沙明显著且呈线性地降低血浆血清素水平。马普替林或安慰剂对血浆血清素无影响。血浆血清素浓度可用于监测氟伏沙明治疗的依从性。
