School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, China.
Department of Immunology, Jiangsu Key Laboratory of Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang 212013, China; Department of Laboratory Medicine, The Affiliated People's Hospital, Jiangsu University, Zhenjiang 212013, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2022 Nov 15;281:121599. doi: 10.1016/j.saa.2022.121599. Epub 2022 Jul 6.
A thiophene substituted naphthyl hydrazone derivative NHT was synthesized using a one-step route for the detection of trivalent chromium (Cr). UV-visible absorption and emission spectra, density functional theory calculations as well as H NMR titration confirmed that the probe underwent a turn-off response via the chelation enhanced fluorescence quenching effect upon exposure to Cr and the NHT-Cr complex was formed at a 1:1 binding stoichiometry. NHT exhibited a fast response rate of 2.3 min in buffer solution and a relatively low limit of detection of 41 nM. In addition, the Schiff base chemosensor exhibited excellent selectivity with high affinity towards Cr in the presence of other competing cations. Bioimaging of the probe in PC3 cells further demonstrated the potential real life application of the probe in detecting Cr.
一种噻吩取代的萘基腙衍生物 NHT 通过一步法合成，用于检测三价铬 (Cr)。紫外-可见吸收和发射光谱、密度泛函理论计算以及 1H NMR 滴定证实，该探针在暴露于 Cr 时通过螯合增强荧光猝灭效应发生关闭响应，并且形成了 NHT-Cr 配合物，其具有 1:1 的结合计量比。NHT 在缓冲溶液中的快速响应速率为 2.3 分钟，并且检测限相对较低，为 41 nM。此外，Schiff 碱化学传感器在存在其他竞争阳离子的情况下，对 Cr 表现出优异的选择性和高亲和力。探针在 PC3 细胞中的生物成像进一步证明了该探针在检测 Cr 方面的潜在实际应用。
