CSIR-Central Leather Research Institute, Adyar, 20, Chennai, Tamil Nadu, India.
Centre for Regenerative Medicine and Stem Cell Research, Sri Ramachandra Institute of Higher Education and Research (DU), Chennai, Tamil Nadu 600116, India.
Colloids Surf B Biointerfaces. 2022 Sep;217:112656. doi: 10.1016/j.colsurfb.2022.112656. Epub 2022 Jun 26.
The present study explores the differentiation of myoblasts in bioengineered 3D composite scaffolds containing keratin and gelatin. Based on the composition and rheological properties three different scaffolds namely HM1, HM2 and HM3 were prepared, characterized and employed for the present study. The scaffolds were then subjected to CC myoblasts differentiation under in vitro conditions as per the standard protocols. Results reveal a wide variation in the elastic modulus, water uptake and degradation rate of the scaffolds significantly impact the myogenesis processes. Composite scaffolds HM2 and HM3 ease the myogenesis compared to HM1, wherein, results in nil myogenesis. Among HM2 and HM3, accelerated myogenesis and the significant expression of myogenin mRNA levels along with extensive myotube development were observed in the HM3 scaffold. In conclusion, scaffolds modulus play a vital role in myogenesis and the observations of the present study provide a possible strategy for better skeletal muscle regeneration using composite scaffolds.
本研究探讨了含有角蛋白和明胶的生物工程 3D 复合支架中肌母细胞的分化。根据组成和流变性质，制备了三种不同的支架，即 HM1、HM2 和 HM3，并对其进行了表征和研究。然后根据标准方案，在体外条件下将支架用于 CC 肌母细胞分化。结果表明，支架的弹性模量、吸水率和降解率差异很大，这些因素显著影响肌生成过程。与 HM1 相比，复合支架 HM2 和 HM3 更有利于肌生成，而 HM1 则没有肌生成。在 HM2 和 HM3 中，HM3 支架中观察到肌生成加速和肌生成因子 mRNA 水平的显著表达以及广泛的肌管发育。总之，支架模量在肌生成中起着至关重要的作用，本研究的观察结果为使用复合支架更好地进行骨骼肌再生提供了一种可能的策略。
