Onyia K A
Comp Immunol Microbiol Infect Dis. 1987;10(1):33-9. doi: 10.1016/0147-9571(87)90038-5.
FMLP was potently chemotactic in vivo to rat leucocytes. Doses of 1 microgram attracted net leucocytes into experimental cotton pellets in 6 h. Maximal chemotactic activity occurred at 0.01 mg. Doses greater or less than 0.01 mg attracted less net leucocytes into experimental pellets. The effect of time on chemotaxis of 0.05 mg FMLP showed that between 3 and 36 h, chemotactic index remained above unity and rose maximally to over 1.5. Maximal chemotactic index occurred at 6 h followed by maximal leucocytic infiltration. FMLP showed early chemoattraction in vivo to rat leucocytes. Leucocytic migration into control pellets rose from 3 h, reached maximum levels at 24 h and remained almost at this level at 36 h. L-methionyl-L-leucyl-L-phenylalanine (MLP), studied as a negative control, showed a chemotactic index and chemotactic differential that rose and fell together between 3 and 36 h. The kinetics of migrating leucocyte populations in response to FMLP showed absolute polymorphonuclear leucocytes at 3 h and over 90% mononuclear leucocytes at 36 h.
N-甲酰甲硫氨酰-亮氨酰-苯丙氨酸（FMLP）在体内对大鼠白细胞具有强烈的趋化作用。1微克的剂量在6小时内可使白细胞净迁移至实验性棉球中。最大趋化活性出现在0.01毫克时。剂量大于或小于0.01毫克时，进入实验性棉球的白细胞净迁移量较少。0.05毫克FMLP的趋化作用随时间的变化表明，在3至36小时之间，趋化指数保持在1以上，并最大升至超过1.5。最大趋化指数出现在6小时，随后白细胞浸润达到最大值。FMLP在体内对大鼠白细胞表现出早期趋化作用。白细胞向对照棉球的迁移从3小时开始增加，在24小时达到最高水平，并在36小时几乎保持在该水平。作为阴性对照进行研究的L-甲硫氨酰-L-亮氨酰-L-苯丙氨酸（MLP），其趋化指数和趋化差异在3至36小时之间同步上升和下降。响应FMLP的迁移白细胞群体动力学显示，3小时时绝对是多形核白细胞，36小时时单核白细胞超过90%。
