Onyia K A
Comp Immunol Microbiol Infect Dis. 1986;9(1):79-88. doi: 10.1016/0147-9571(86)90078-0.
A new in vivo method for studying chemotaxis and leucocyte migration was developed in male Wistar rats, using the well characterized chemoattractant, N-formyl-L-methionyl-L-phenylalanine (FMP) as positive control, and dental cotton pellets. 2.0 mg FMP attracted the greatest net leucocytes into test pellets, while doses higher or lower, attracted less cells. Use of L-methionyl-L-leucyl-L-phenylalanine (MLP) as negative control showed that the method could be used to distinguish chemotactic migrations from other migrations. The kinetics of the leucocytic infiltration showed the predominance of polymorphonuclear leucocytes at the early reactions and the arrival of mononuclear leucocytes in later stages. Use of Staphylococcal protein A and trypsin demonstrated that it was possible to assay the activities of those chemoattractants which require complement activation for chemotaxis. However, complement was not normally activated by the technique. The method requires simple surgery, seems a good model for evaluating the in vivo applicability of the numerous in vitro chemotactic findings, and should possibly be used to develop agents such as drugs destined to produce changes in the inflammatory process.
在雄性Wistar大鼠中开发了一种用于研究趋化性和白细胞迁移的新体内方法，使用特征明确的趋化因子N-甲酰-L-蛋氨酰-L-苯丙氨酸（FMP）作为阳性对照，并使用牙科棉粒。2.0毫克FMP吸引到测试棉粒中的白细胞净数量最多，而更高或更低剂量吸引的细胞较少。使用L-蛋氨酰-L-亮氨酰-L-苯丙氨酸（MLP）作为阴性对照表明，该方法可用于区分趋化性迁移与其他迁移。白细胞浸润的动力学表明，在早期反应中多形核白细胞占优势，而在后期单核白细胞到达。使用葡萄球菌蛋白A和胰蛋白酶表明，可以测定那些需要补体激活才能产生趋化性的趋化因子的活性。然而，该技术通常不会激活补体。该方法需要简单的手术，似乎是评估众多体外趋化性研究结果体内适用性的良好模型，并且可能应该用于开发诸如旨在改变炎症过程的药物等制剂。
