Department of Oral Medicine, Hebei Eye Hospital, Xiangdu District, Xingtai, Hebei Province, China.
Cell Mol Biol (Noisy-le-grand). 2022 Feb 27;67(6):305-310. doi: 10.14715/cmb/2021.67.6.40.
The first mandible and maxilla permanent molars are the first permanent teeth that grow next to the deciduous teeth and may decay due to carelessness. Their caries can spread to the pulpal tentacles and cause pulpal and periapical diseases. In the current study, we tried to compare the curative effect of different retrograde filling materials, i.e. white MTA, gray MTA, Portland cement, and IRM, in young permanent molar root canal therapy. Because IL-1β stimulates bone degradation by osteoclasts, IL-1β gene expression was also measured for further evaluation. For this purpose, 400 students (240 boys and 160 girls) aged 8 to 11 years referred to the Pediatric Dental Center for first permanent molar root canal therapy were selected during two years. After recording the demographic characteristics of each patient, the first permanent molar teeth were examined by a general dentist with Abslang and decayed teeth were considered to have both discolorations in their grooves and apparent opacity. The patients, who need root canal therapy, were divided into four groups. The first group was treated with gray MTA. The second group was treated with white MTA. The third group received Portland cement for root canal therapy. The fourth group was treated with IRM. Also, IL-1β gene expression was evaluated by the real-time PCR technique. Relative changes in gene expression in PBMC cells were performed using One Way ANOVA. SPSS 18 software was used to determine the correlation of gene expression in PBMCs. The results showed that there was no significant difference between the groups in terms of age (p = 0.12) and gender (p = 0.24). Also, the need for endodontic treatment in the mandible (n = 278) was higher than the maxilla (n = 85) and both jaws (n = 37). But there was no significant difference between the groups in terms of the need for endodontic treatment (p = 0.32). The results of Pearson correlation coefficients between studied groups in terms of IL-1β gene expression showed that gray MTA and white MTA were not statistically different, but MTAs were generally different from Portland cement and IRM, with higher IL-1β gene expression. In general, the results showed that the teeth in the vicinity of gray MTA and white MTA showed a more appropriate response than Portland cement and IRM, so the use of MTA and its preference over other materials is recommended. In the case of Portland cement, more studies are needed to reach a conclusion comparing this material with MTA.
下颌和上颌第一恒磨牙是继乳牙之后萌出的第一副恒牙，由于护理不当，可能会发生龋坏。其龋坏可扩散至牙髓角，引起牙髓和根尖周疾病。在目前的研究中，我们试图比较不同逆行充填材料（即白色 MTA、灰色 MTA、波特兰水泥和 IRM）在年轻恒牙根管治疗中的疗效。因为 IL-1β 通过破骨细胞刺激骨降解，所以还测量了 IL-1β 基因表达以进行进一步评估。为此，在两年内，从儿科牙科中心选择了 400 名 8 至 11 岁的学生（240 名男孩和 160 名女孩）进行第一恒磨牙根管治疗。在记录每位患者的人口统计学特征后，由一名全科牙医使用 Abslang 检查第一恒磨牙，患有龋齿的牙齿在其凹槽中既有变色，又有明显的不透明。需要根管治疗的患者被分为四组。第一组用灰色 MTA 治疗。第二组用白色 MTA 治疗。第三组接受波特兰水泥根管治疗。第四组用 IRM 治疗。此外，还通过实时 PCR 技术评估了 IL-1β 基因表达。使用 One Way ANOVA 分析 PBMC 细胞中基因表达的相对变化。使用 SPSS 18 软件确定 PBMC 中基因表达的相关性。结果表明，在年龄（p = 0.12）和性别（p = 0.24）方面，各组之间无显著差异。此外，下颌（n = 278）需要根管治疗的病例高于上颌（n = 85）和上下颌（n = 37）。但各组之间在根管治疗的需要方面无显著差异（p = 0.32）。在 IL-1β 基因表达方面，研究组之间的 Pearson 相关系数表明，灰色 MTA 和白色 MTA 之间没有统计学差异，但 MTA 通常与波特兰水泥和 IRM 不同，IL-1β 基因表达更高。总的来说，结果表明灰色 MTA 和白色 MTA 附近的牙齿比波特兰水泥和 IRM 表现出更适当的反应，因此建议使用 MTA 并优先选用该材料。在波特兰水泥的情况下，需要进行更多的研究以得出将该材料与 MTA 进行比较的结论。
