First Department of Cardiovascular, Hebei General Hospital, Hebei 050001, P.R. China.
Department of Cardiovascular, 4th Hospital of Hebei Medical University, Hebei 050011, P.R. China.
Cell Mol Biol (Noisy-le-grand). 2022 Feb 4;67(5):324-331. doi: 10.14715/cmb/2021.67.5.44.
To study the clinical significance of miR-27a expression in serum exosomes in patients with heart failure (HF), this study was carried out. Totally 101 chronic heart failure (CHF) patients (research group) admitted to our hospital from April 2016 to January 2019 were enrolled, and another 30 healthy subjects (control group) who underwent physical examination during the same period were selected. The difference of miR-27a expression level in serum exosomes between the two groups of subjects was compared, so as to analyze the diagnostic value of miR-27a in CHF and the relationship between miR-27a expression level and patient prognosis. The expression level of miR-27a in peripheral blood exosomes of subjects in the control group (CG) was significantly higher than that in the research group (RG) (P<0.05), while the miR-27a expression of the latter increased significantly 3 months after treatment (P<0.05). ROC analysis showed that the AUC, sensitivity, specificity, and critical level of miR-27a in the diagnosis of CHF were 0.835, 77.23%, 80.00%, and 1.060 respectively. While after 3 months of treatment, the indicators of the RG effectively improved (P<0.05). Pearson and Sparman correlation analysis revealed that there was a significant linear correlation between miR-27a and each indicator (P<0.05). K-M survival curve analysis demonstrated that the survival rate of patients with high expression of miR-27a was significantly higher than that of patients with low expression (P<0.05). The expression of mR-27a in serum exosomes is decreased in patients with HF and has important potential value in the diagnosis and prognosis of CHF.
为了研究心力衰竭（HF）患者血清外泌体中 miR-27a 表达的临床意义，进行了这项研究。本研究纳入了 2016 年 4 月至 2019 年 1 月期间我院收治的 101 例慢性心力衰竭（CHF）患者（研究组），并选择同期体检的 30 名健康受试者作为对照组。比较两组受试者血清外泌体中 miR-27a 表达水平的差异，分析 miR-27a 在 CHF 中的诊断价值及其表达水平与患者预后的关系。对照组（CG）受试者外周血外泌体中 miR-27a 的表达水平明显高于研究组（RG）（P<0.05），而后者治疗 3 个月后 miR-27a 的表达明显升高（P<0.05）。ROC 分析显示，miR-27a 诊断 CHF 的 AUC、敏感度、特异度和临界值分别为 0.835、77.23%、80.00%和 1.060。而治疗 3 个月后，RG 的各项指标均有效改善（P<0.05）。Pearson 和 Sparman 相关分析显示，miR-27a 与各指标呈显著线性相关（P<0.05）。K-M 生存曲线分析表明，miR-27a 高表达患者的生存率明显高于低表达患者（P<0.05）。HF 患者血清外泌体中 mR-27a 的表达降低，对 CHF 的诊断和预后具有重要的潜在价值。
