Swindell R T, Bell V C, Slaughter S, Albers-Jackson B
Curr Eye Res. 1987 Mar;6(3):451-6. doi: 10.3109/02713688709025201.
Rabbit lenses were incubated in organ culture with 14C-galactose for 6, 12 and 20 hours. Gangliosides were extracted using the Folch-Suzuki method, purified by dialysis and analyzed by thin-layer chromatography. Six radioactive bands, including the origin, were observed. Tentative identification of these bands as N-acetylneuraminylgalactosylglucosylceramide (GM3), N-acetylgalactosaminyl-(N-acetylneuraminyl)-galactosylglucosylceramide (GM2), galactosyl-N-acetylgalactosaminyl-(N-acetylneuraminyl)- galactosylglucosylceramide (GM1), N-acetylneuraminylgalactosyl-N- acetylgalactosaminyl-(N-acetylneuraminyl)-galactosylglucosylceramide (GD1a), N-acetylneuraminylgalactosyl-N-acetylgalactosaminyl-(N- acetylneuraminyl-N-acetylneuraminyl)-galactosylglucosylceramide (GT) was made by comparison with authentic standard gangliosides. Galactose incorporation into GM3 and GM2 increased during the first 12 hours but decreased during the period from 12 to 20 hours. GD1a and GT incorporated the greatest amount of label during the period from 12 to 20 hours. Incorporation of labeled galactose into GM1 was nearly constant during this time period. Specific activities for GM1, GM3 and GT were nearly the same at 6 hours and were about half those of GM2 and GD1a for the same time period.
将兔晶状体置于器官培养中，与¹⁴C - 半乳糖一起孵育6小时、12小时和20小时。采用Folch - Suzuki法提取神经节苷脂，通过透析纯化，并进行薄层层析分析。观察到包括原点在内的六条放射性条带。通过与标准神经节苷脂对照，初步鉴定这些条带分别为N - 乙酰神经氨酰半乳糖基葡糖神经酰胺（GM3）、N - 乙酰半乳糖胺基 -（N - 乙酰神经氨酰）- 半乳糖基葡糖神经酰胺（GM2）、半乳糖基 - N - 乙酰半乳糖胺基 -（N - 乙酰神经氨酰）- 半乳糖基葡糖神经酰胺（GM1）、N - 乙酰神经氨酰半乳糖基 - N - 乙酰半乳糖胺基 -（N - 乙酰神经氨酰）- 半乳糖基葡糖神经酰胺（GD1a）、N - 乙酰神经氨酰半乳糖基 - N - 乙酰半乳糖胺基 -（N - 乙酰神经氨酰 - N - 乙酰神经氨酰）- 半乳糖基葡糖神经酰胺（GT）。在最初的12小时内，半乳糖掺入GM3和GM2的量增加，但在12至20小时期间减少。GD1a和GT在12至20小时期间掺入的标记量最大。在此时间段内，标记半乳糖掺入GM1的量几乎保持恒定。GM1、GM3和GT的比活性在6小时时几乎相同，而在同一时间段内，它们约为GM2和GD1a的比活性的一半。
