Reid D M, Forrester J V, Campbell A M
Curr Eye Res. 1987 Apr;6(4):561-8. doi: 10.3109/02713688709025214.
The naturally occurring humoral immune response of Campbell and Hunter strains of Royal College of Surgeons (RCS) rat to bovine retinal S-antigen and bovine rod outer segments was tested by a sensitive ELISA (enzyme linked immunosorbent assay) in animals up to the age of 9 weeks. Controls of Piebald Virol Glaxo, Wistar and DA rats were also employed. The ELISA results indicate that rats of all strains had a response to both antigens. The magnitude of the response in any particular strain varied with the age of the animal and the antigen and serum dilution employed in the assay. Considerable variation in response was observed among animals of the same strain at any one particular age. Immunoblot analysis of the strongest ELISA positive sera detected S-antigen and other autoantigenic proteins in both RCS and Wistar rats. The data suggest that the antibody response observed may have little significance to the retinal degeneration observed with RCS rats.
通过灵敏的酶联免疫吸附测定（ELISA），检测了皇家外科学院（RCS）大鼠的坎贝尔和亨特品系在9周龄以内的动物对牛视网膜S抗原和牛视杆外段的天然体液免疫反应。还使用了花斑维罗尔·葛兰素、Wistar和DA大鼠作为对照。ELISA结果表明，所有品系的大鼠对两种抗原均有反应。任何特定品系的反应强度随动物年龄、测定中使用的抗原和血清稀释度而变化。在任何一个特定年龄，同一品系的动物之间观察到反应有相当大的差异。对ELISA最强阳性血清的免疫印迹分析在RCS和Wistar大鼠中均检测到了S抗原和其他自身抗原蛋白。数据表明，观察到的抗体反应可能与RCS大鼠所观察到的视网膜变性关系不大。
