一种用于快速筛选植物病原体-生物防治剂的离体叶片测定法。
A Detached Leaf Assay for Rapidly Screening Plant Pathogen-Biological Control Agents.
作者信息
Macan Giovana Prado Fortuna, Khalil Sammar, Kalyandurg Pruthvi B, Pareek Nidhi, Vetukuri Ramesh R
机构信息
Department of Plant Breeding, Swedish University of Agricultural Sciences, Alnarp, Sweden.
Department of Biosystems and Technology, Swedish University of Agricultural Sciences, Alnarp, Sweden.
出版信息
Methods Mol Biol. 2022;2536:449-458. doi: 10.1007/978-1-0716-2517-0_26.
The detached leaf assay (DLA) is a nondestructive method for evaluating interactions between plants and disease-causing agents that allows quick characterization of potential pathogens' infectivity and plants' resistance to them. Here we show its utility for also assessing potential biological control agents (BCAs), by demonstrating its applicability for screening potential BCAs for the strawberry (Fragaria × ananassa) pathogen Phytophtora cactorum.
离体叶片测定法（DLA）是一种评估植物与致病因子之间相互作用的非破坏性方法，它能够快速鉴定潜在病原体的感染力以及植物对它们的抗性。在此，我们通过证明其适用于筛选草莓（Fragaria × ananassa）病原体恶疫霉（Phytophtora cactorum）的潜在生物防治剂（BCA），展示了该方法在评估潜在生物防治剂方面的效用。
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