Department of Nutrition and Food Science, University of Maryland, College Park, Maryland 20742, United States.
Methods and Application of Food Composition Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, United States Department of Agriculture, Beltsville, Maryland 20705, United States.
J Agric Food Chem. 2022 Jul 27;70(29):9039-9047. doi: 10.1021/acs.jafc.2c03418. Epub 2022 Jul 12.
The soluble free, soluble conjugated, and insoluble bound phenolic compounds in tomato seeds were extracted and analyzed using ultra-high-performance liquid chromatography-high-resolution mass spectrometry. Total phenolic content (TPC) and free radical scavenging activities along with the antiproliferative effects against the human colorectal cancer cell line (HCT-116) were also examined for the soluble free, soluble conjugated, and insoluble bound phenolic fractions. 13, 7, and 10 compounds were tentatively identified in the soluble free, soluble conjugated, and insoluble bound phenolic fractions, respectively, including indole-3-acetic acid derivatives, flavonoids, phenolic acid, and tyramine-derived hydroxycinnamic acid amines. The insoluble bound phenolic fraction was observed to have a greater TPC value and stronger free radical scavenging activities against ABTS, DPPH, and peroxyl radicals and a stronger inhibitory effect against HCT-116 cells compared with the soluble free and the soluble conjugated fractions. Soluble free and insoluble bound fractions significantly inhibited the proliferation of the HCT-116 cell line, and no antiproliferative effects were observed with the soluble conjugated fraction under the experimental conditions. The results may provide a foundation for future application of tomato seeds as nutraceuticals in dietary supplements and functional foods.
采用超高效液相色谱-高分辨质谱联用技术，对番茄籽中的游离态可溶、结合态可溶和不溶结合酚类化合物进行了提取和分析。同时，还检测了游离态可溶、结合态可溶和不溶结合酚类化合物的总酚含量（TPC）和自由基清除活性，以及对人结肠癌细胞（HCT-116）的增殖抑制作用。在游离态可溶、结合态可溶和不溶结合酚类化合物中，分别暂定鉴定出了 13、7 和 10 种化合物，包括吲哚-3-乙酸衍生物、类黄酮、酚酸和酪胺衍生的羟基肉桂酰基胺。与游离态可溶和结合态可溶部分相比，不溶结合酚类化合物具有更高的 TPC 值和更强的 ABTS、DPPH 和过氧自由基清除活性，以及对 HCT-116 细胞更强的抑制作用。游离态可溶和不溶结合部分显著抑制了 HCT-116 细胞系的增殖，而结合态可溶部分在实验条件下则没有观察到增殖抑制作用。这些结果为未来将番茄籽作为膳食补充剂和功能性食品中的营养保健品应用提供了基础。
