Barutta Federica, Bellini Stefania, Guarrera Simonetta, Matullo Giuseppe, Schalkwijk Casper, Stehouwer Coen D, Chaturvedi Nish, Soedamah-Muthu Sabita S, Durazzo Marilena, Gruden Gabriella
Department of Medical Sciences, University of Turin, Italy.
Department of Medical Sciences, University of Turin, Italy.
Diabetes Res Clin Pract. 2022 Aug;190:109987. doi: 10.1016/j.diabres.2022.109987. Epub 2022 Jul 9.
To investigate whether serum miR-145-5p levels were associated with micro-macrovascular chronic complications in patients with type 1 diabetes (DM1).
A nested case-control study from the EURODIAB Prospective Complications Study was performed. Cases (n = 289) had one or more complications of diabetes, whereas controls (n = 153) did not have any complication. We measured miR-145-5p levels by qPCR and investigated the association with diabetes complications.
Mean miR-145-5p levels were significantly lower in cases with microangiopathy [2.12 (0.86-4.94)] compared to controls [3.15 (1.21-7.36), P < 0.05] even after adjustment for age, gender, and diabetes duration. In logistic regression analysis, miR-145-5p levels in the lowest tertile were associated with an over three-fold increased odds ratio (OR) of albuminuria [3.22 (1.17-8.81)], independently of both demographic and diabetes-related factors. In addition, mir145-5p levels in the lowest tertile were independently and inversely associated with arterial hypertension [1.96 (1.08-3.56)] and hypertension was the mediator of the relationship between miR-145-5p and albuminuria.
In this large cohort of DM1 patients, we found an inverse association between miR-145-5p and albuminuria that was mediated by systemic hypertension.
探究1型糖尿病（DM1）患者血清miR-145-5p水平是否与微血管和大血管慢性并发症相关。
开展一项来自欧洲糖尿病前瞻性并发症研究的巢式病例对照研究。病例组（n = 289）有一项或多项糖尿病并发症，而对照组（n = 153）无任何并发症。我们通过定量聚合酶链反应（qPCR）测量miR-145-5p水平，并研究其与糖尿病并发症的关联。
即使在调整年龄、性别和糖尿病病程后，微血管病变患者的平均miR-145-5p水平[2.12（0.86 - 4.94）]仍显著低于对照组[3.15（1.21 - 7.36），P < 0.05]。在逻辑回归分析中，最低三分位数的miR-145-5p水平与蛋白尿的比值比（OR）增加三倍以上相关[3.22（1.17 - 8.81）]，且独立于人口统计学和糖尿病相关因素。此外，最低三分位数的mir145-5p水平与动脉高血压独立且呈负相关[1.96（1.08 - 3.56）]，并且高血压是miR-145-5p与蛋白尿之间关系的中介因素。
在这个大型DM1患者队列中，我们发现miR-145-5p与蛋白尿之间存在由系统性高血压介导的负相关。
