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骨髓间充质干细胞来源的外泌体通过调节 5/6 肾切除大鼠的 klotho 来保护肾脏免受损伤。

BMSC-derived exosomes protect against kidney injury through regulating klotho in 5/6 nephrectomy rats.

机构信息

Department of Nephrology, Hangzhou TCM Hospital Affiliated to Zhejiang Chinese Medical University, Tiyuchang Road 453, Hangzhou, 31007, People's Republic of China.

Key Laboratory of Kidney Disease Prevention and Control Technology, Hangzhou, Zhejiang, China.

出版信息

Eur J Med Res. 2022 Jul 11;27(1):118. doi: 10.1186/s40001-022-00742-8.

DOI:10.1186/s40001-022-00742-8
PMID:35820962
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9277829/
Abstract

AIM

The aim of this study was to investigate the renoprotective effects of exosomes derived from rat bone marrow mesenchymal stem cells (rBMSCs) in a rat model of 5/6 nephrectomy (Nx)-induced chronic kidney disease (CKD).

METHODS

A rat model of 5/6 Nx-induced CKD was established using conventional method. rBMSC-derived exosomes were isolated using ultracentrifugation and characterized. The exosomes were injected into 5/6 Nx rats through the caudal vein. After 12 weeks, 24 h proteinuria, serum creatinine (SCr), and blood urea nitrogen (BUN) levels were evaluated, and renal pathology was analyzed by H&E and Masson staining, and transmission electron microscopy. The expression of klotho was analyzed and the activity of the klotho promoter was evaluated using a luciferase reporter assay.

RESULTS

The isolated exosomes showed typical morphological features. Exosomes transplantation reduced 24 h urinary protein excretion, and SCr and BUN levels in 5/6 Nx-induced CKD rats. Furthermore, renal pathology was improved in the exosome-treated 5/6 Nx rats. Mechanistically, the exosomes significantly upregulated the activity of klotho promoter and its expression.

CONCLUSIONS

Transplantation of rBMSC-derived exosomes may protect against kidney injury, probably by regulating klotho activity and expression. Our results provide a theoretical basis for the application of rBMSC-derived exosomes in CKD therapy.

摘要

目的

本研究旨在探讨大鼠骨髓间充质干细胞（rBMSC）来源的外泌体在 5/6 肾切除（Nx）诱导的慢性肾脏病（CKD）大鼠模型中的肾保护作用。

方法

采用常规方法建立 5/6 Nx 诱导的 CKD 大鼠模型。采用超速离心法分离 rBMSC 来源的外泌体，并进行鉴定。通过尾静脉将外泌体注射到 5/6 Nx 大鼠体内。12 周后，评估 24 小时蛋白尿、血清肌酐（SCr）和血尿素氮（BUN）水平，并通过 H&E 和 Masson 染色以及透射电镜分析肾脏病理。分析 klotho 的表达，并通过荧光素酶报告基因检测评估 klotho 启动子的活性。

结果

分离的外泌体显示出典型的形态特征。外泌体移植可减少 5/6 Nx 诱导的 CKD 大鼠的 24 小时尿蛋白排泄、SCr 和 BUN 水平。此外，外泌体处理的 5/6 Nx 大鼠的肾脏病理得到改善。机制上，外泌体显著上调了 klotho 启动子的活性及其表达。

结论

rBMSC 来源的外泌体移植可能通过调节 klotho 的活性和表达来保护肾脏免受损伤。我们的研究结果为 rBMSC 来源的外泌体在 CKD 治疗中的应用提供了理论依据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/862c/9277829/d96925df3c7e/40001_2022_742_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/862c/9277829/d087684f2b5c/40001_2022_742_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/862c/9277829/ddfa65f13c86/40001_2022_742_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/862c/9277829/dbce76dcd2ac/40001_2022_742_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/862c/9277829/1e5f74702b8c/40001_2022_742_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/862c/9277829/d96925df3c7e/40001_2022_742_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/862c/9277829/d087684f2b5c/40001_2022_742_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/862c/9277829/ddfa65f13c86/40001_2022_742_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/862c/9277829/dbce76dcd2ac/40001_2022_742_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/862c/9277829/1e5f74702b8c/40001_2022_742_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/862c/9277829/d96925df3c7e/40001_2022_742_Fig5_HTML.jpg

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Pharmazie. 2017 Aug 1;72(8):468-474. doi: 10.1691/ph.2017.7525.
运动诱导的细胞外囊泡以运动强度依赖的方式介导人结肠癌细胞的凋亡。
Eur J Appl Physiol. 2025 Apr 20. doi: 10.1007/s00421-025-05787-1.
4
Mesenchymal stem cell-derived extracellular vesicles for human diseases.间充质干细胞衍生的细胞外囊泡用于人类疾病治疗
Extracell Vesicles Circ Nucl Acids. 2024 Feb 6;5(1):64-82. doi: 10.20517/evcna.2023.47. eCollection 2024.
5
Frontier role of extracellular vesicles in kidney disease.细胞外囊泡在肾脏疾病中的前沿作用。
J Nanobiotechnology. 2024 Sep 20;22(1):583. doi: 10.1186/s12951-024-02852-3.
6
Extracellular Vesicles Derived from Human Liver Stem Cells Counteract Chronic Kidney Disease Development and Cardiac Dysfunction in Remnant Kidney Murine Model: The Possible Involvement of Proteases.源自人肝干细胞的细胞外囊泡可对抗残余肾小鼠模型中的慢性肾病发展和心脏功能障碍：蛋白酶的可能作用。
Biomedicines. 2024 Jul 8;12(7):1517. doi: 10.3390/biomedicines12071517.
7
Naïve or Engineered Extracellular Vesicles from Different Cell Sources: Therapeutic Tools for Kidney Diseases.来自不同细胞来源的天然或工程化细胞外囊泡：肾脏疾病的治疗工具。
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8
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9
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