Centre for Ecological and Evolutionary Synthesis (CEES), Department of Biosciences and the Department of Immunology, University of Oslo, Oslo, Norway.
Section for Genetics and Evolutionary Biology, Department of Biosciences and the Department of Immunology, University of Oslo, Oslo, Norway.
Commun Biol. 2022 Jul 11;5(1):689. doi: 10.1038/s42003-022-03645-w.
Atlantic Cod (Gadus morhua) has lost the major histocompatibility complex class II presentation pathway. We recently identified CD8-positive T cells, B cells, and plasma cells in cod, but further characterisation of lymphocyte subsets is needed to elucidate immune adaptations triggered by the absence of CD4-positive T lymphocytes. Here, we use single-cell RNA sequencing to examine the lymphocyte heterogeneity in Atlantic cod spleen. We describe five T cell subsets and eight B cell subsets and propose a B cell trajectory of differentiation. Notably, we identify a subpopulation of T cells that are CD8-negative. Most of the CD8-negative T lymphocytes highly express the homologue of monocyte chemotactic protein 1b, and another subset of CD8-negative T lymphocytes express the homologue of the scavenger receptor m130. Uncovering the multiple lymphocyte cell sub-clusters reveals the different immune states present within the B and T cell populations, building a foundation for further work.
大西洋鳕鱼（Gadus morhua）已经失去了主要组织相容性复合体 II 类呈递途径。我们最近在鳕鱼中鉴定出了 CD8 阳性 T 细胞、B 细胞和浆细胞，但需要进一步鉴定淋巴细胞亚群，以阐明由 CD4 阳性 T 淋巴细胞缺失引发的免疫适应。在这里，我们使用单细胞 RNA 测序来研究大西洋鳕鱼脾脏中的淋巴细胞异质性。我们描述了五个 T 细胞亚群和八个 B 细胞亚群，并提出了 B 细胞分化轨迹。值得注意的是，我们鉴定出了一群 CD8 阴性的 T 细胞。大多数 CD8 阴性 T 淋巴细胞高度表达单核细胞趋化蛋白 1b 的同源物，另一群 CD8 阴性 T 淋巴细胞表达清道夫受体 m130 的同源物。揭示多种淋巴细胞细胞亚群揭示了 B 和 T 细胞群体中存在的不同免疫状态，为进一步的工作奠定了基础。
