Khatib Thabat, Müller Berndt, McCaffery Peter
School of Medicine, Medical Sciences and Nutrition, Institute of Medical Sciences, University of Aberdeen, Aberdeen, Scotland, UK.
Methods Mol Biol. 2022;2524:197-207. doi: 10.1007/978-1-0716-2453-1_15.
The present protocol describes a bioluminescence reporter assay developed to quantify the ability of synthetic agonists of retinoic acid receptors (RARs) to activate glutamate receptor subunit 1 (GluR1) translation. The reporter assay uses firefly luciferase under the control of the GluR1 5' untranslated region (5' UTR) which is bound by RARs to regulate its translation. This method is used to demonstrate the role of RARα in retinoic acid regulation of GluR1 translation. This method may also be used to screen drugs that influence RAR induction of GluR1 translation as an important mechanism controlling learning and memory in the brain.
本实验方案描述了一种生物发光报告基因检测方法，该方法用于量化视黄酸受体（RARs）的合成激动剂激活谷氨酸受体亚基1（GluR1）翻译的能力。该报告基因检测方法使用在GluR1 5'非翻译区（5' UTR）控制下的萤火虫荧光素酶，RARs与该区域结合以调节其翻译。此方法用于证明RARα在视黄酸调节GluR1翻译中的作用。该方法还可用于筛选影响RAR诱导GluR1翻译的药物，这是控制大脑学习和记忆的重要机制。
