Kumar Sudheeran Pradeep, Kumari B D Ranjitha
Department of Postharvest Science of Fresh Produce, Agricultural Research Organization (ARO), The Volcani Center, P.O. Box 15159, HaMaccabim Road 68, Rishon Lezion 7505101, Israel.
Department of Botany, Bharathidasan University, Tiruchirappalli 620024, India.
BioTech (Basel). 2021 Aug 21;10(3):18. doi: 10.3390/biotech10030018.
The present investigation aimed to obtain salt-tolerant L. to develop a constant form through in vitro mutagenesis with ethyl methane sulphonate (EMS) as the chemical mutagen. NaCl tolerance was evaluated by the ability of the callus to maintain its growth under different concentrations, ranges from (0 mM to 500 mM). However, NaCl salinity concentration at (500 mM) did not show any development of callus, slight shrinking, and brown discoloration taking place over a week. Thus, all the biochemical and antioxidant assays were limited to (0-400 mM) NaCl. On the other hand, selected calluses were treated with 0.5% EMS for 30, 60, and 90 min and further subcultured on basal media fortified with different concentrations of 0-400 mM NaCl separately. Thus, the callus was treated for 60 min and was found to induce the mutation on the callus. The maximum salt-tolerant callus from 400 mM NaCl was regenerated in MS medium fortified with suitable hormones. Biochemical parameters such as chlorophyll, carotenoids, starch, amino acids, and phenol contents decreased under NaCl stress, whereas sugar and proline increased. Peroxidase (POD) and superoxide dismutase (SOD) activities peaked at 200 mM NaCl, whereas catalase (CAT) was maximum at 100 mM NaCl. Enhanced tolerance of 0.5% the EMS-treated callus, attributed to the increased biochemical and antioxidant activity over the control and NaCl stress. As a result, the mutants were more tolerant of salinity than the control plants.
本研究旨在获得耐盐的番茄植株，通过使用甲基磺酸乙酯（EMS）作为化学诱变剂进行体外诱变来培育稳定的株系。通过愈伤组织在不同浓度（0 mM至500 mM）下维持生长的能力来评估其耐NaCl能力。然而，在500 mM的NaCl盐浓度下，愈伤组织未出现任何生长，一周内出现轻微萎缩和褐色变色。因此，所有生化和抗氧化分析均限于0 - 400 mM的NaCl浓度范围。另一方面，选择的愈伤组织分别用0.5%的EMS处理30、60和90分钟，然后在添加不同浓度（0 - 400 mM）NaCl的基础培养基上进一步继代培养。结果发现，处理60分钟的愈伤组织发生了诱变。在添加合适激素的MS培养基中再生出了来自400 mM NaCl处理的最大耐盐愈伤组织。在NaCl胁迫下，叶绿素、类胡萝卜素、淀粉、氨基酸和酚类物质等生化参数下降，而糖和脯氨酸增加。过氧化物酶（POD）和超氧化物歧化酶（SOD）活性在200 mM NaCl时达到峰值，而过氧化氢酶（CAT）在100 mM NaCl时活性最高。0.5% EMS处理的愈伤组织耐受性增强，这归因于其生化和抗氧化活性相对于对照和NaCl胁迫有所增加。结果，突变体比对照植株更耐盐。
