Department of Botany, Savitribai Phule Pune University, Pune, India.
Department of Environmental Science, Savitribai Phule Pune University, Pune, India.
Sci Rep. 2019 Aug 29;9(1):12522. doi: 10.1038/s41598-019-48737-6.
Spinach (Spinacia oleracea L.) is a vegetable plant with high nutritional properties. In the present work, we studied responses of in vitro shoot cultures to salt stress (0 (control), 100, 200 and 300 mM NaCl) and salt stress-induced accumulation of 20-hydroxyecdysone (20E). Our results revealed that effect of low to moderate level of salinity stress (100-200 mM) was less pronounced on growth and tissue water content (TWC) of shoot cultures compared to higher salinity level (300 mM). The salt treated shoot cultures showed better osmotic adjustment in terms of significant accumulation of compatible solutes and total soluble sugars and also higher antioxidant enzyme activity. As the NaCl stress was increased, there was a corresponding linear raise in the Na accumulation while the contents of both K and Ca decreased significantly. We also studied salt-stress induced accumulation of a bioactive compound; 20E and results showed that 200 mM salt treated shoot cultures accumulated significantly 2.9 fold higher 20E as compared to untreated shoot cultures. The results suggest that Spinacia oleracea exhibits considerable salt tolerance with better osmotic adjustment and can be considered a suitable candidate for the production of bioactive secondary metabolite.
菠菜（Spinacia oleracea L.）是一种具有高营养价值的蔬菜植物。在本工作中，我们研究了体外芽培养物对盐胁迫（0（对照）、100、200 和 300 mM NaCl）和盐胁迫诱导的 20-羟基蜕皮甾酮（20E）积累的反应。我们的结果表明，与高盐水平（300 mM）相比，低至中度盐胁迫（100-200 mM）对芽培养物的生长和组织水含量（TWC）的影响较小。盐处理的芽培养物在渗透调节方面表现出更好的效果，表现为相容性溶质和总可溶性糖的显著积累，以及更高的抗氧化酶活性。随着 NaCl 胁迫的增加，Na 的积累呈线性增加，而 K 和 Ca 的含量则显著下降。我们还研究了盐胁迫诱导生物活性化合物；20E 的积累，结果表明，与未处理的芽培养物相比，200 mM 盐处理的芽培养物积累了 2.9 倍更高的 20E。结果表明，Spinacia oleracea 表现出相当的耐盐性，具有更好的渗透调节能力，可以考虑作为生物活性次生代谢产物生产的合适候选物。
