Mallick Prashant K, Sindhania Ankita, Gupta Toshi, Singh Dhirendra P, Saini Seema, Singh Om P
National Institute of Malaria Research, New Delhi, India.
Centre for Genomics, Jiwaji University, Gwalior, India.
Med Vet Entomol. 2023 Jun;37(2):209-212. doi: 10.1111/mve.12596. Epub 2022 Jul 13.
There are at least three known knockdown resistance (kdr) mutations reported globally in the human head louse Pediculus humanus capitis De Geer (Phthiraptera: Anoplura) that are associated with reduced sensitivity to pyrethroids. However, the prevalence of kdr mutation in head lice is not known in the Indian subcontinent. To identify kdr mutations in the Indian head lice population, the genomic region of the voltage-gated sodium channel gene encompassing IIS1-2 linker to IIS6 segments was PCR-amplified and sequenced from P. humanus capitis samples collected from different geographic localities of India. DNA sequencing revealed the presence of four kdr mutations: M827I, T929I, L932F and L1014F. The presence of a classical kdr mutation L1014F, the most widely reported mutation across insect-taxa associated with the kdr-trait, is being reported for the first time in P. humanus capitis.
在全球范围内，至少有三种已知的击倒抗性（kdr）突变在人头虱（Pediculus humanus capitis De Geer，虱目：吸虱亚目）中被报道，这些突变与对拟除虫菊酯的敏感性降低有关。然而，在印度次大陆，人头虱中kdr突变的流行情况尚不清楚。为了鉴定印度人头虱种群中的kdr突变，对包含从印度不同地理区域收集的人头虱样本中电压门控钠通道基因从IIS1-2连接子到IIS6片段的基因组区域进行了PCR扩增和测序。DNA测序揭示了四种kdr突变的存在：M827I、T929I、L932F和L1014F。经典的kdr突变L1014F是昆虫类群中报道最广泛的与kdr性状相关的突变，首次在人头虱中被报道。
