Holmes G L, Thompson J L, Smeyne R J, Wallace R B
Epilepsia. 1987 May-Jun;28(3):242-50. doi: 10.1111/j.1528-1157.1987.tb04214.x.
Transplantation of embryonic tissue into the brains of host animals has been demonstrated to totally or partially correct functional lesions and neurohormonal deficits in a variety of animals. This study evaluated the hypothesis that "naive" embryonic neural tissue implanted into previously kindled animals will alter subsequent seizure susceptibility. At age 16 days, male rats were electrically kindled in the right amygdala (AM). Following kindling (age 19 days), rats underwent either transplants of embryonic neocortical tissue into the left dorsal hippocampus or a sham procedure. At age 84 days, both groups underwent transfer kindling in the left AM. In addition, two other groups of 19-day-old pups that had had electrode implantation in the right AM without subsequent kindling received either embryonic neocortical implants in the left dorsal hippocampus or a sham procedure and were kindled in the left AM for the first time at age 84 days. There were no differences in rate of kindling between the animals that received successfully grafted transplants and those that underwent sham procedures. Using the kindling model, transplantation of embryonic neocortical tissue into the hippocampus does not significantly alter seizure susceptibility in the host animal.
将胚胎组织移植到宿主动物脑内已被证明能完全或部分纠正多种动物的功能性损伤和神经激素缺陷。本研究评估了“未经处理的”胚胎神经组织植入先前已点燃的动物体内会改变其后续癫痫易感性的假说。16日龄时，雄性大鼠在右侧杏仁核进行电点燃。点燃后（19日龄），大鼠接受将胚胎新皮质组织移植到左侧背侧海马体或假手术。84日龄时，两组均在左侧杏仁核进行转移点燃。此外，另外两组19日龄幼犬，右侧杏仁核植入电极但未后续点燃，分别接受左侧背侧海马体胚胎新皮质植入或假手术，并在84日龄首次在左侧杏仁核点燃。成功接受移植的动物与接受假手术的动物在点燃速率上没有差异。利用点燃模型，将胚胎新皮质组织移植到海马体不会显著改变宿主动物的癫痫易感性。
