巴西利什曼原虫：体外对数期与稳定期前鞭毛体之间的蛋白质、碳水化合物及抗原差异
Leishmania braziliensis: protein, carbohydrate, and antigen differences between log phase and stationary phase promastigotes in vitro.
作者信息
Grögl M, Franke E D, McGreevy P B, Kuhn R E
出版信息
Exp Parasitol. 1987 Jun;63(3):352-9. doi: 10.1016/0014-4894(87)90183-4.
When Leishmania species are grown in vitro, parasites from the stationary phase differ from those in log phase growth in being more infective and more resistant to complement and macrophage mediated killing. In the present study, log phase and stationary phase promastigotes of Leishmania braziliensis panamensis were compared at the molecular level. Differences in polypeptide and glycoprotein composition and antigenicity between log and stationary phase promastigotes of L. b. panamensis were detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting; the former showed that two polypeptides were unique to log phase promastigotes and one was unique to stationary phase promastigotes. There were also differences in surface lectin binding characteristics of log and stationary phase promastigotes. Live stationary phase promastigotes bound more concanavalin and lentil lectin than log phase promastigotes, indicating a greater number of mannose residues on their surfaces.
当利什曼原虫在体外培养时，稳定期的寄生虫与对数期生长的寄生虫不同，前者更具感染性，对补体和巨噬细胞介导的杀伤更具抵抗力。在本研究中，对巴西利什曼原虫巴拿马亚种的对数期和稳定期前鞭毛体进行了分子水平的比较。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和蛋白质印迹法检测了巴拿马亚种利什曼原虫对数期和稳定期前鞭毛体之间多肽、糖蛋白组成和抗原性的差异；前者显示有两种多肽是对数期前鞭毛体特有的，一种是稳定期前鞭毛体特有的。对数期和稳定期前鞭毛体的表面凝集素结合特性也存在差异。活的稳定期前鞭毛体比对数期前鞭毛体结合更多的伴刀豆球蛋白和扁豆凝集素，表明其表面有更多的甘露糖残基。
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