Smith P J, Warren R M, von Holt C
FEBS Lett. 1987 May 11;215(2):305-10. doi: 10.1016/0014-5793(87)80167-9.
The biotin analogue biotinylglycyltyrosine has been synthesized and labelled to a specific activity of 2000 Ci/mmol with 125I. This analogue has been used in conjunction with immobilized streptavidin in an assay which detects as little as 1 fmol biotin or biotinylated molecules in solution. The determination of biotinylated insulin in a tissue extract and the quantitation of a transcription assay are given as examples.
生物素类似物生物素基甘氨酰酪氨酸已被合成，并用¹²⁵I标记至比活度为2000 Ci/mmol。该类似物已与固定化链霉亲和素一起用于一种检测溶液中低至1 fmol生物素或生物素化分子的分析方法中。文中给出了测定组织提取物中生物素化胰岛素以及转录分析定量的示例。
