Mock D M, DuBois D B
Anal Biochem. 1986 Mar;153(2):272-8. doi: 10.1016/0003-2697(86)90092-8.
Interest in accurate measurement of biotin concentrations in plasma and urine has been stimulated by recent advances in the understanding of biotin-responsive inborn errors of metabolism and by several reports describing acquired biotin deficiency during parenteral alimentation. This paper presents a biotin assay utilizing radiolabeled avidin in a sequential, solid-phase method; the assay has increased sensitivity compared to previous methods (greater than or equal to 10 fmol/tube), correlates with expected trends in biotin concentrations in blood and urine in a rat model of biotin deficiency, and can utilize commercially available radiolabeled avidin.
对生物素反应性先天性代谢缺陷认识的最新进展以及几篇描述胃肠外营养期间获得性生物素缺乏的报告，激发了人们对准确测量血浆和尿液中生物素浓度的兴趣。本文介绍了一种利用放射性标记抗生物素蛋白的顺序固相生物素检测方法；与以前的方法相比，该检测方法的灵敏度有所提高（大于或等于10 fmol/管），与生物素缺乏大鼠模型中血液和尿液中生物素浓度的预期趋势相关，并且可以使用市售的放射性标记抗生物素蛋白。
