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在缺乏钠泵三个亚基的溶藻弧菌突变体中,钠泵的结合依赖性恢复。

Conjugation-dependent recovery of the Na+ pump in a mutant of Vibrio alginolyticus lacking three subunits of the Na+ pump.

作者信息

Tokuda H, Udagawa T, Asano M, Yamamoto T, Unemoto T

出版信息

FEBS Lett. 1987 May 11;215(2):335-8. doi: 10.1016/0014-5793(87)80173-4.

DOI:10.1016/0014-5793(87)80173-4
PMID:3582659
Abstract

The Na+ pump-deficient mutant, Nap1, of Vibrio alginolyticus was found to lack three subunits of Na+-dependent NADH:quinone oxidoreductase complex. Although a spontaneous Na+ pump positive revertant did not appear from Nap1, transconjugants that recovered both the Na+ pump activity and the subunits were isolated from Nap1 conjugated with the wild type. Moreover, the wild type was found to contain two different sizes of plasmids. These results suggest the possibility that the Na+ pump is encoded by a plasmid.

摘要

溶藻弧菌的钠离子泵缺陷型突变体Nap1被发现缺乏钠离子依赖的NADH:醌氧化还原酶复合物的三个亚基。尽管Nap1未出现自发的钠离子泵阳性回复突变体,但从与野生型接合的Nap1中分离出了恢复了钠离子泵活性和亚基的转接合子。此外,发现野生型含有两种不同大小的质粒。这些结果表明钠离子泵可能由质粒编码。

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FEBS Lett. 1987 May 11;215(2):335-8. doi: 10.1016/0014-5793(87)80173-4.
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引用本文的文献

1
Respiratory Na+ pump and Na+-dependent energetics in Vibrio alginolyticus.溶藻弧菌中的呼吸性钠离子泵及钠离子依赖性能量代谢
J Bioenerg Biomembr. 1989 Dec;21(6):693-704. doi: 10.1007/BF00762687.