Beattie P, Tan K, Bourne R M, Leach D, Rich P R, Ward F B
Institute for Cell and Molecular Biology, Edinburgh University, UK.
FEBS Lett. 1994 Dec 19;356(2-3):333-8. doi: 10.1016/0014-5793(94)01275-x.
Oligonucleotide probes based on the N-terminal amino acid sequences of the NqrA and NqrC subunits were used to clone genes for the Na(+)-dependent NADH-ubiquinone oxidoreductase complex from Vibrio alginolyticus. Four consecutive ORFs were identified encoding subunit proteins of 48.6, 46.8, 27.7 and 22.6 kDa, respectively (NqrA-D). A further ORF, showing 71% homology to the BolA protein of Escherichia coli, was located upstream. From sequence comparisons, we conclude that the Na(+)-dependent NADH-ubiquinone oxidoreductase complex of V. alginolyticus is clearly distinct from the corresponding H(+)-dependent enzymes of both prokaryotes and eukaryotes.
基于溶藻弧菌NqrA和NqrC亚基N端氨基酸序列的寡核苷酸探针，被用于克隆该菌中依赖Na⁺的NADH-泛醌氧化还原酶复合物的基因。鉴定出四个连续的开放阅读框，分别编码48.6、46.8、27.7和22.6 kDa的亚基蛋白（NqrA-D）。在其上游还定位了一个与大肠杆菌BolA蛋白有71%同源性的开放阅读框。通过序列比较，我们得出结论，溶藻弧菌中依赖Na⁺的NADH-泛醌氧化还原酶复合物与原核生物和真核生物中相应的依赖H⁺的酶明显不同。
