通过流式细胞术对抗体分泌细胞系进行细胞周期分析。
Cell cycle analysis by flow cytometry in an antibody secreting cell line.
作者信息
Schliermann M, Beckers C, Miltenburger H G
出版信息
Dev Biol Stand. 1987;66:101-9.
PMID:3582741
Abstract
This presentation reports on the routine use of flow cytometric techniques in suspension cultures. As a model system an antibody secreting mouse hybridoma cell line was used to monitor antibody production (a Bromodeoxyuridine specific antibody) and proliferative status as obtained by flow cytometry (FCM) and cell counting.
摘要
本报告介绍了流式细胞术技术在悬浮培养中的常规应用。作为一个模型系统，使用了一种分泌抗体的小鼠杂交瘤细胞系来监测抗体产生（一种溴脱氧尿苷特异性抗体）以及通过流式细胞术（FCM）和细胞计数获得的增殖状态。
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