Biochemistry of hybridoma technology.

The study of the metabolic requirements of cells in vitro requires both a qualitative definition of such nutrients and a quantitative analysis to optimize cell growth. The development of a rapid weaning procedure which allows cells to be cultured in low serum concentrations, facilitates the easy assessment of factors which regulate cell growth and antibody productivity. The novel combination of existing technology; the MTT (tetrazolium) cell ELISA (Mosman, 1983) and the TSP (Transferable solid phase) immuno ELISA provides a method for studying such factors.