Kanamori A, Kagawa H, Nagahama Y
Gen Comp Endocrinol. 1987 May;66(2):210-7. doi: 10.1016/0016-6480(87)90269-3.
Highly purified 125I-labeled chum salmon, Oncorhynchus keta, gonadotropin (GTH; CSG-SII) demonstrated specific binding to membrane fractions prepared from amago salmon (Oncorhynchus rhodurus) postovulatory ovary. Previously these ovaries had been reported to produce steroid hormones in response to GTH stimulation. Maximum specific binding occurred at pH 7 to 8 after 18-24 hr of incubation at 4 degrees in the presence of 40 mM Ca2+. As the concentration of labeled hormone increased, specific binding to the membrane preparation of the postovulatory ovary approached saturation. NIH ovine LH and FSH partially inhibited the specific binding of CSG-SII to the GTH receptors, but chum salmon growth hormone, prolactin, and alpha-MSH did not displace the binding. These results provide evidence for the existence of specific and saturable GTH receptors in the postovulatory ovary of amago salmon.
高度纯化的125I标记的大麻哈鱼（Oncorhynchus keta）促性腺激素（GTH；CSG - SII）显示出与从红点鲑（Oncorhynchus rhodurus）排卵后卵巢制备的膜部分有特异性结合。此前有报道称，这些卵巢在GTH刺激下会产生类固醇激素。在4℃下，于40 mM Ca2 +存在的情况下孵育18 - 24小时后，最大特异性结合发生在pH 7至8时。随着标记激素浓度的增加，排卵后卵巢膜制剂的特异性结合接近饱和。美国国立卫生研究院（NIH）的绵羊促黄体生成素（LH）和促卵泡生成素（FSH）部分抑制了CSG - SII与GTH受体的特异性结合，但大麻哈鱼生长激素、催乳素和α - 促黑素细胞激素（α - MSH）并未取代这种结合。这些结果为红点鲑排卵后卵巢中存在特异性和可饱和的GTH受体提供了证据。
