Yasuda A, Miyazima K, Kawauchi H, Peter R E, Lin H R, Yamaguchi K, Sano H
Gen Comp Endocrinol. 1987 May;66(2):280-90. doi: 10.1016/0016-6480(87)90278-4.
The complete amino acid sequence of common carp (Cyprinus carpio) prolactin (PRL) has been determined. One milligram of carp PRL was reduced, carboxymethylated, and then cleaved with cyanogen bromide and several enzymes. Another milligram of carp PRL was digested with lysyl endopeptidase. The resulting fragments were separated by reverse-phase high-performance liquid chromatography and subjected to sequence analysis by the automatic Edman method with a gas-liquid sequencer and the manual DNS-Edman method. The carp PRL consists of 186 amino acid residues with two disulfide linkages formed between residues 46-160 and 177-186. In addition, it lacks the linkage in the amino-terminal portion of mammalian prolactins, as do salmon and tilapia PRLs, and shows 77% sequence identity with salmon PRL and 36% identity with mammalian PRLs. The sequence comparison has been performed with 190 alignment positions. Four conserved segments of the alignment positions 1-32, 46-66, 71-94, and 155-182 that were found between salmon and mammalian PRLs are further confirmed in carp PRL. Variant residues are clustered in the region of residues 98-143 which has 48% sequence identity with salmon PRL and only 2% with mammalian PRL.
鲤科鱼（鲤鱼）催乳素（PRL）的完整氨基酸序列已被确定。将1毫克鲤鱼催乳素进行还原、羧甲基化处理，然后用溴化氰和几种酶进行切割。另1毫克鲤鱼催乳素用赖氨酰内肽酶消化。所得片段通过反相高效液相色谱法分离，并采用气液测序仪通过自动埃德曼法和手动DNS-埃德曼法进行序列分析。鲤鱼催乳素由186个氨基酸残基组成，在第46 - 160位残基和第177 - 186位残基之间形成两个二硫键。此外，与鲑鱼和罗非鱼的催乳素一样，它在哺乳动物催乳素的氨基末端部分缺少这种连接，并且与鲑鱼催乳素的序列同一性为77%，与哺乳动物催乳素的同一性为36%。序列比较是在190个比对位置上进行的。在鲑鱼和哺乳动物催乳素之间发现的比对位置1 - 32、46 - 66、71 - 94和155 - 182的四个保守区段在鲤鱼催乳素中得到进一步证实。变异残基聚集在第98 - 143位残基区域，该区域与鲑鱼催乳素的序列同一性为48%，与哺乳动物催乳素的序列同一性仅为2%。
